B59 or B-59 may refer to:
- B-59 military hardware:
- Boeing XB-59, American aircraft model
- Soviet submarine B-59, a submarine
- B59:
- European highways:
- Bundesstraße 59, in the German Bundesstraße system
- Eisenstädter Straße in the Austrian Landesstraße system
- Volvo B59 Swedish-market bus chassis
- HLA-B59, serotype re Human Leucocyte Antigen
- B59, chess variation arising from 5...Nc6 in Sicilian Defence
- Barnard 59, AKA Pipe Nebula
- European highways:
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