The women's 4 × 100 metres relay event at the 2003 Pan American Games was held on August 9.
Results edit
| Rank | Nation | Athletes | Time | Notes |
|---|---|---|---|---|
 |
 United States |
Angela Williams, Consuella Moore, Angela Daigle, Lauryn Williams | 43.06 | |
 |
 Cuba |
Dainelky Pérez, Roxana Díaz, Virgen Benavides, Misleidys Lazo | 43.40 | |
 |
 Jamaica |
Lacena Golding-Clarke, Judyth Kitson, Shellene Williams, Danielle Browning | 43.71 | |
| 4 |  Trinidad and Tobago |
Keenan Gibson, Fana Ashby, Wanda Hutson, Kelly-Ann Baptiste | 43.97 | NR |
| 5 |  Colombia |
Melisa Murillo, Digna Luz Murillo, Patricia Rodríguez, Norma González | 45.13 | |
| 6 |  Dominican Republic |
Nelsy Delgado, María Carrión, Elizabeth Balbuena, Marleni Mejía | 45.76 | NR |
 Bahamas |
DNS |