Appuleia Varilla (flourished 1st century AD) was a Roman noblewoman and the only daughter of Sextus Appuleius, consul in 29 BC. Tacitus describes her as the grand-niece of the emperor Augustus. Ronald Syme reports that an inscription from Asia confirms she was the daughter of that consul and the sister (actually she would be the daughter of his sister Quinctilia) of Publius Quinctilius Varus.
In 17, she was charged by Tiberius with adultery and insulting the Roman empress (his mother Livia Drusilla) and his step-father Augustus. In the trial she was acquitted of treason, but her adultery was punished. She was exiled from Rome and ordered not to come within 200 miles of the city. This punishment by relatives was traditional in this circumstance. Manlius, her lover, was banned from Italy and Africa Province.