The 2009 UniCredit Czech Open was a tennis clay-court tournament included in the 2009 ATP Challenger Tour. Agustín Calleri was the defending singles champion, but could not get past the first round. Jan Hájek, world no. 288, came from the qualifying round and took the title in front of his home crowd by beating the top-100 player Steve Darcis in the final. Johan Brunström and Jean-Julien Rojer won the doubles event.
|2009 UniCredit Czech Open|
|Date||June 1–June 6|
|Johan Brunström / Jean-Julien Rojer|
- Rankings are as of May 25, 2009.
The following players received wildcards into the singles main draw:
The following players received entry a special Exempt into the singles main draw:
The following players received entry from the qualifying draw: