James Macfarlane may refer to:
- James Macfarlane (Tasmanian politician) (1844–1914), member of the Australian Senate for Tasmania
- James Macfarlane (Western Australian politician) (1865–1942), member of the Western Australian Legislative Council
- James Macfarlane (moderator) (1808–1866), Scottish minister and ecclesiastical author
- James MacFarlane (1866–1942), New Zealand cricketer
See also
edit- James Macfarlan (1832–1862), Scottish poet
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