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Summary
| Description |
English: Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches for fragments that are methylated (IP fraction). This IP fraction can become the input sample to one of two DNA detection methods: array hybridization using high-density microarrays (A) or high-throughput sequencing using the latest in sequencing technology (B). Output from these methods are then analyzed for methylation patterns to answer the biological question. |
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This file was derived from: Medip-final-3.jpg:  |
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This is a retouched picture, which means that it has been digitally altered from its original version. Modifications: svg. The original can be viewed here: Medip-final-3.jpg: .
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Licensing
I, the copyright holder of this work, hereby publish it under the following license:
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I, the copyright holder of this work, release this work into the public domain. This applies worldwide. In some countries this may not be legally possible; if so: I grant anyone the right to use this work for any purpose, without any conditions, unless such conditions are required by law. |
Original upload log
This image is a derivative work of the following images:
- File:Medip-final-3.jpg licensed with PD-self
- 2009-03-03T06:16:21Z Gavinha 2268x1868 (357388 Bytes) {{Information |Description={{en|1=Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches
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File history
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 05:11, 15 June 2013 | | 1,150 × 1,000 (648 KB) | Shakiestone | == {{int:filedesc}} == {{Information |Description={{en|1=Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches for... |
File usage
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