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Summary
| Description |
English: This figure outlines the process of fluorescence in situ hybridization (FISH) used for bacterial pathogen identification. First, a sample of the infected tissue is taken from the patient. Then an oligonucleotide that is complementary to the suspected pathogen’s genetic code is synthesized and chemically tagged with a fluorescent probe. The collected tissue sample must then be chemically treated in order to make the cell membranes permeable to the fluorescently tagged oligonucleotide. After the tissue sample is treated, the tagged complementary oligonucleotide is added. The fluorescently tagged oligonucleotide will only bind to the complementary DNA of the suspected pathogen. If the pathogen is present in the tissue sample, then the pathogen’s cells will glow/fluoresce after treatment with the tagged oligonucleotide. All other cells will not glow after treatment. |
| Date | |
| Source |
"File:Micropipeta icona.png". Link: File:Micropipeta_icona.png ; "File:Microtube.png". Link: https://commons.wikimedia.org/wiki/File:Microtube.png ; "File:Microtube2.png". Link: File:Microtube2.png ; "File: Optical Microscope.png". Link: File:Optical_Microscope.png ; "File:Reagenzglas.svg". Link:https://commons.wikimedia.org/wiki/File:Reagenzglas.svg ; "File:3ml vector syringe 2point9ml.svg". Link: https://commons.wikimedia.org/wiki/File:3ml_vector_syringe_2point9ml.svg ; |
| Author |
Pepetps Togopic Ivan Akira Magnus Manske Timothy W. Ford |
Links to works used:
File:Micropipeta_icona.png ;
File:Microtube.png ;
File:Microtube2.png ;
File:Optical_Microscope.png ;
File:Reagenzglas.svg ;
File:3ml_vector_syringe_2point9ml.svg ;
Names of authors of works used (in order of works listed above): Pepetps Togopic Ivan Akira Magnus Manske Timothy W. Ford
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File history
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 01:30, 18 December 2016 | | 2,000 × 1,125 (785 KB) | Matheny6 | User created page with UploadWizard |
