File:Citrus tern cb simplified.svg

Original file(SVG file, nominally 899 × 911 pixels, file size: 121 KB)

Summary

Description
English: Citrus fruits clustered by genetic similarity of partial sequences. Ternary diagram showing hybridization in citrus cultivars. Data from (2014-12). "Next generation haplotyping to decipher nuclear genomic interspecific admixture in Citrusspecies: analysis of chromosome 2". BMC Genetics 15 (1). DOI:10.1186/s12863-014-0152-1. ISSN 1471-2156. Retrieved on 2017-07-08.
Date
Source Own work
Author HLHJ
Other versions File:Citrus_tern_cb_simplified_1.svg (updated version using full, not partial, sequences) This version should be used. The original paper plots a slightly extended version of this superseded dataset in 3-D, see File:S12863-014-0152-1-3.gif.

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Derivation

This diagram was made using the Unix command line, the statistics language R, and the SVG editor Inkscape, using the following algorithm. Use "Edit" to get it with newlines, and thus formatted so you can cut-and-paste it.

Script for making the diagram from the raw data

#Data from Additional file 8, Curk et al., "Next generation haplotyping to decipher nuclear genomic interspecific admixture in Citrus species: analysis of chromosome 2" BMC Genetics 2014 15:152, doi:10.1186/s12863-014-0152-1
#Copy-and-paste pdf's table to an ASCII text file, citrus_tern_table.txt
#Remove the error estimates by removing all lines beginning with '±'

cat citrus_tern_table.txt | sed '/^±/ d' > citrus_tern_table2.txt

#Remove blank lines

cat citrus_tern_table2.txt | sed '/^$/ d' > citrus_tern_table3.txt

#Because sed reads things line-by-line, replacing patterns containing newlines (\n0 with \t0, in this case) is awkward.
#Thanks http://stackoverflow.com/questions/1251999/how-can-i-replace-a-newline-n-using-sed?noredirect=1

cat citrus_tern_table3.txt | sed -e ':a' -e 'N' -e '$!ba' -e 's/\n0/\t0/g' > citrus_tern_table4.txt

#Add a leading newline back, so you get a leading tab

echo -e | cat - citrus_tern_table4.txt > citrus_tern_table5.txt

#Replace the newlines in the header with tabs

cat citrus_tern_table5.txt | sed -e ':a' -e 'N' -e '$!ba' -e 's/\nC\. /\tC\./g' > citrus_tern_table_done.txt

#Manually took out a random newline in "Deep Red [\n]pummelo"
#Manually changed Meyer lemo -> Meyer lemon
#Manually changed Nanfengmijuu -> Nanfengmiju
#Enter R command-line environment

R

#Load needed library. If fails, may need to run this: install.packages("ggtern")

library(ggtern)

#read datatable and tidy format

citrus_table = read.csv("./citrus_tern_table_done.txt",header=TRUE, sep = "\t") dimnames(citrus_table)1<-citrus_table[,1] citrus_table[,1] <- NULL

#====================================================================
#Make an original theme that works for colourblind viewers
#See http://bconnelly.net/2013/10/creating-colorblind-friendly-figures/

colours_colourblind<-c("black"="#000000", "cb_orange"="#E69F00", "cb_pale_blue"="#56B4E9", "cb_green"="#009E73", "cb_yellow"="#F0E442", "cb_darkblue"="#0072B2", "cb_red"="#D55E00", "cb_purple"="#CC79A7")

theme_colourblind<-theme_custom(base_size = 12, base_family = "", tern.plot.background = NULL, tern.panel.background = "grey92", col.T = colours_colourblind[2], col.L = colours_colourblind[6], col.R = colours_colourblind[7], col.grid.minor = "white")

#====================================================================
#Basic plot using built-in theme, not too good for colourblind viewers, for debugging
#citrus_tern <- ggtern(data = citrus_table, aes(x = C.maxima, y = C.reticulata, z = C.medica)) +geom_point(size = 4, shape = 21, color = "black") + ggtitle("Citrus hybrids") + theme_gray() + theme(legend.position = c(0,1), legend.justification = c(0, 1)) +geom_text(aes(label=rownames(citrus_table)),hjust=0, vjust=0)
#Plot that works for colourblind viewers,

citrus_tern <- ggtern(data = citrus_table, aes(x = C.maxima, y = C.reticulata, z = C.medica)) +geom_point(size = 4, shape = 21, color = "black") + ggtitle("Citrus hybrids") + theme_colourblind + theme(legend.position = c(0,1), legend.justification = c(0, 1)) +geom_text(aes(label=rownames(citrus_table)),hjust=0, vjust=0)

citrus_tern

#====================================================================
#Output plot as an svg file

svg("citrus_tern.svg",width=10,height=10) citrus_tern dev.off()

#Prettify. Manually edit labels, titles, etc. using the Inkscape SVG editor

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8 July 2017

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Date/TimeThumbnailDimensionsUserComment
current01:10, 9 July 2017Thumbnail for version as of 01:10, 9 July 2017899 × 911 (121 KB)HLHJDistinguishes between lemons (asexually propagated bud sport group), and recent independent hybrids, now labeled ''Brand "lemon"'' rather than ''Brand lemon''.
16:45, 8 July 2017Thumbnail for version as of 16:45, 8 July 2017899 × 911 (119 KB)HLHJUser created page with UploadWizard
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