Talk:Reduced representation bisulfite sequencing

Latest comment: 10 years ago by Partridgefoot in topic MspI cut site

Orphan Link edit

This technique is an evolution of the older technique where we use sodium bilsufite to modify unprotected Cytosines into Uracil (Cytosines can be protected with Methyl-groups). This technique was developed in 2009, I think. See this link to the scientific paper: Smith, Z. D., Gu, H., Bock, C., Gnirke, A. & Meissner, A. High-throughput bisulfite sequencing in mammalian genomes. Methods 48, 226–232 (2009).

I am however unsure where to find the bisulfite technique... 84.112.136.52 (talk) 14:48, 2 November 2013 (UTC) Edit: Found it, linked to it http://en.wikipedia.org/wiki/Bisulfite_sequencing - not sure if my formatting is proper, but at least the content is valid IMO. I also removed the orphan-notice because we now have a link to the "parent" technique in the article. 84.112.136.52 (talk) 14:51, 2 November 2013 (UTC)Reply

MspI cut site edit

The cut site of MspI given here is wrong. It is 5'CCGG3', not 3'CCGG5'. See New England Biolabs MspI page. — Preceding unsigned comment added by Partridgefoot (talkcontribs) 17:11, 1 April 2014 (UTC)Reply