Talk:Nick translation

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Er edit

Latest comment: 15 years ago2 comments2 people in discussion

Er, how kan the Klenow Pol I fragment translate from a nick onwards? The Klenow fragment doesn't have 5' 3' exonuclease activity, hence it cannot remove the downstream nucleotides.

And a question concerning the second part: when DNase I nicks the dsDNA strand, it does so at random places, thus on both sense and antisense strands. But during polymerisation by Pol I, doesn't the progressive 5' 3' exonuclease eventually lead to a ds break? (Of course assuming that there is a nick downstream from the first, but on the other strand.) 84.197.145.92 16:33, 3 June 2007 (UTC) (Jack the Stripper until he gets his nickname back)Reply

Yes, DS breaks will occur. However, they do not pose problems in subsequent hybridisations since the two smaller fragments will still both hybridise to their respective target sequences (at least until the DNA is excessively degraded into <30bp fragments, which does not occur in practice). 137.224.252.10 (talk) 14:21, 29 October 2008 (UTC)Reply

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