Talk:Lysis

Removed from article

I have removed the following from the article: ==The dissolving of [[cell (biology)|cells]] sometimes known more specifically as [[cytolysis]] == Lysis - For easily disrupted cells such as insect and animal cells grown in culture media, a mild method for cell disruption (lysis) is commonly used. Quite frequently, simply lowering the ionic strength of the media will cause the cells to swell and burst. In some cases it is also desirable to add a mild surfactant and some mild mechanical agitation or sonication to completely disassociate the cellular components. Due to the cost and relative effort to grow these cells, there is often only a small quantity of cells to be processed, and preferred methods for cell disruption tend to be a manual mechanical homogenizer, nitrogen burst methods, or ultrasound with a small probe. Because these methods are performed under very mild conditions, they are often used for subcellular fractionation studies. For cells that are more difficult to disrupt, such as bacteria, yeast, and algae, hypotonic shock alone generally is insufficient to open the cell and stronger methods must be used. These organisms have cell walls that must be broken to allow access to intracellular components. == The reduction of [[symptom]]s of a [[disease]] == . AndyZ 01:41, 9 February 2006 (UTC)

Applications

"It also helps the immune system and there are about 60 in each cell". Someone please rewrite this to say 60 of WHAT. Thank you. Sys Hax 03:22, 11 December 2006 (UTC)

Yes, please. I came to the discussion page to make the same request. 75.80.131.76 05:34, 25 January 2007 (UTC)

Plasmolysis

Now how is that bursting a cell? I'll change the definition to fit the content. Jack the Stripper 18:53, 18 July 2007 (UTC)

External links modified

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Assessment comment

The comment(s) below were originally left at Talk:Lysis/Comments, and are posted here for posterity. Following several discussions in past years, these subpages are now deprecated. The comments may be irrelevant or outdated; if so, please feel free to remove this section.

Could we move this page together with centrifugation techniques so the all the basics are in one place. I don't know how much more there is to say about lysate. It's a crushed cell and the first part of a handful of techniques. --Fapril (talk) 20:50, 22 April 2009 (UTC)

Substituted at 18:28, 17 July 2016 (UTC)

External links modified

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