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Stainless steel - bad material choice edit
Stainless steel threads tend to gall up as soon as any pressure is applied. If you use stainless steel make either the bolt nor the nut of a differing material, using stainless steel for both will permanently cold weld them together.
AVE on youtube, he's crude but this gets the point across, https://www.youtube.com/watch?v=mHAhfX7iRjs
On some high precision gage fixtures I've made, I used O6 tool steel, O6 is like more common O2 oil hardening tool steel but with graphite flakes distributed within, and A2 air hardening tool steel. Lapped after heat treatment, this combination is silky smooth like a micrometer screw should be.