Talk:Diethyl pyrocarbonate

Latest comment: 17 years ago by Confuseddave in topic Untitled

Untitled edit

DEPC treated (and therefore RNase-free) water is used in all handling of RNA in the laboratory, to reduce the risk of RNA being degraded by RNases.

All? Ironic that this statement comes just after a paragraph describing the conditions under which DEPC cannot be used.

Confuseddave 11:47, 13 February 2007 (UTC)Reply

I have found resource according to which the increased concentration (1% instead of usual 0.1%) of DEPC can also be used for Tris solutions. Also, on the same site they write to add Tris separately, so I really do not understand which is the right way...

       dmanagadze