Talk:Barnase

Latest comment: 11 years ago by 79.158.109.229 in topic Updated complex

Updated complex edit

1brs seems to be outdated.. there is at least one higher resolution structure for the barnase-barstar complex (1ay7). Feel free to update. Zargulon 14:55, 26 September 2006 (UTC)Reply

--79.158.109.229 (talk) 15:07, 4 September 2012 (UTC) I can read English but I write it very bad and I don't know if this have any interest. I have read at the open access article "Profile of B. Golberg" of PNAS 109 (8)2688-2690 the following information about barnase: "The team set out to engineer male sterility in tobacco, the plant in which Goldberg first discovered the anther-specific genes. Goldberg's laboratory cloned the promoter of one of those genes, TA29, and Mariani fused it to a self-defense gene from the soil bacterium Bacillus amyloliquefaciens called barnase, which encodes a ribonuclease. The researchers inserted the construct into the tobacco plant, where the cytotoxic RNase specifically destroyed the anther cells during development. The engineered plants were identical to controls except they failed to shed pollen, resulting in male sterility. The team then demonstrated the transferability of the TA29–barnase system to other plants by using it to engineer sterility in canola (15)". It's about pioneering studies in crop engineering.If anybody think that it can be useful...Reply