The Ski complex is a multi-protein complex involved in the 3' end degradation of messenger RNAs in yeast.[1]
Structure
editThe complex consists of three main proteins, the RNA helicase Ski2 and the proteins Ski3 and Ski8. This tetramer contains a 370 kDa core complex, containing N-terminal arms and C-terminal arms from Ski3. The helicase core of Ski2 is positioned by both the C-terminal of Ski3 and two subunits of Ski8.
Mechanism
editHelicase activities are initiated by the N-terminal arm and the Ski2 insertion domain.[2] In yeast, the complex guides RNA molecules to the exosome complex for degradation via a fourth protein, called Ski7, which contains a GTPase-like protein.[3] Ski7 involves the 3’ to 5’ degradation of RNA through two different pathways, 3’ poly(A) tail shortening and the binding of the Ski2, Ski3, and Ski8 tetramer and the exosome.[1]
Degradation of the 3' mRNA overhang occurs by association with the 80s ribosome. The 3' end of the mRNA is threaded through the ribosome to Ski2, preparing it for the degradation process. [4]
Biochemical studies also show that the Ski complex can thread RNA through the exosome complex, thereby coupling the Ski2 protein helicase function with the exoribonuclease activity, leading to degradation of the RNA strand.[2]
See also
editReferences
edit- ^ a b Araki Y, Takahashi S, Kobayashi T, Kajiho H, Hoshino S, Katada T (September 2001). "Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast". The EMBO Journal. 20 (17): 4684–4693. doi:10.1093/emboj/20.17.4684. PMC 125587. PMID 11532933.
- ^ a b Halbach F, Reichelt P, Rode M, Conti E (August 2013). "The yeast ski complex: crystal structure and RNA channeling to the exosome complex". Cell. 154 (4): 814–826. doi:10.1016/j.cell.2013.07.017. PMID 23953113. S2CID 12699297.
- ^ Wang L, Lewis MS, Johnson AW (August 2005). "Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p". RNA. 11 (8): 1291–1302. doi:10.1261/rna.2060405. PMC 1370812. PMID 16043509.
- ^ Schmidt C, Kowalinski E, Shanmuganathan V, Defenouillère Q, Braunger K, Heuer A, et al. (December 2016). "The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex". Science. 354 (6318): 1431–1433. Bibcode:2016Sci...354.1431S. doi:10.1126/science.aaf7520. PMID 27980209. S2CID 30262907.