RLO may refer to:
- Het Rijnlands Lyceum Oegstgeest, a secondary school in the town of Oegstgeest in South Holland
- Right-to-left override, the Unicode control character
- Valle del Conlara Airport, the IATA code RLO
- Radio Limerick One, a licensed radio station serving Limerick city and county
- Rickettsia-like organisms (RLOs)
This disambiguation page lists articles associated with the title RLO.
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