Prohibitin

Prohibitin, also known as PHB, is a protein that in humans is encoded by the PHB gene. The Phb gene has also been described in animals, fungi, plants, and unicellular eukaryotes. Prohibitins are divided in two classes, termed Type-I and Type-II prohibitins, based on their similarity to yeast PHB1 and PHB2, respectively. Each organism has at least one copy of each type of prohibitin gene.

PHB1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1LU7
Identifiers
Aliases	PHB1, prohibitin, HEL-S-54e, HEL-215, PHB, prohibitin 1
External IDs	OMIM: 176705 MGI: 97572 HomoloGene: 1980 GeneCards: PHB1
Gene location (Human) Chr. Chromosome 17 (human) Band 17q21.33 Start 49,404,049 bp End 49,414,905 bp
Gene location (Mouse) Chr. Chromosome 11 (mouse) Band 11 59.01 cM|11 D Start 95,557,783 bp End 95,571,599 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in rectum islet of Langerhans minor salivary glands right adrenal gland left ventricle left adrenal gland body of stomach gastrocnemius muscle stromal cell of endometrium ganglionic eminence Top expressed in yolk sac proximal tubule internal carotid artery interventricular septum external carotid artery sternocleidomastoid muscle lip facial motor nucleus myocardium of ventricle right ventricle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function histone deacetylase binding complement component C3a binding protein C-terminus binding proteinase activated receptor binding complement component C3b binding protein binding enzyme binding DNA-binding transcription factor activity, RNA polymerase II-specific Cellular component cytoplasm membrane myelin sheath plasma membrane integral component of plasma membrane nucleoplasm cell surface early endosome mitochondrion mitochondrial inner membrane extracellular exosome nucleus postsynaptic density mitochondrial crista extrinsic component of mitochondrial outer membrane extrinsic component of presynaptic active zone membrane glutamatergic synapse GABA-ergic synapse Biological process regulation of apoptotic process negative regulation of glucocorticoid receptor signaling pathway DNA biosynthetic process regulation of transcription, DNA-templated cellular response to interleukin-6 negative regulation of protein catabolic process negative regulation of transcription by competitive promoter binding protein stabilization negative regulation of androgen receptor signaling pathway mitochondrion organization negative regulation of transcription by RNA polymerase II positive regulation of transcription, DNA-templated positive regulation of G protein-coupled receptor signaling pathway negative regulation of cell growth osteoblast differentiation positive regulation of ERK1 and ERK2 cascade positive regulation of complement activation negative regulation of ERK1 and ERK2 cascade progesterone receptor signaling pathway negative regulation of transcription, DNA-templated histone deacetylation signal transduction negative regulation of cell population proliferation positive regulation of gene expression positive regulation of cell death mitochondrial calcium ion transmembrane transport ovarian follicle development ovarian follicle atresia animal organ regeneration response to cytokine response to immobilization stress negative regulation of apoptotic process response to peptide hormone response to ethanol Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5245 18673 Ensembl ENSG00000167085 ENSMUSG00000038845 UniProt P35232 P67778 RefSeq (mRNA) NM_002634 NM_001281496 NM_001281497 NM_001281715 NM_008831 RefSeq (protein) NP_001268425 NP_001268426 NP_001268644 NP_002625 NP_032857 Location (UCSC) Chr 17: 49.4 – 49.41 Mb Chr 11: 95.56 – 95.57 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Discovery

Prohibitins are evolutionarily conserved genes that are ubiquitously expressed. The human prohibitin gene, located on the BRCA1 chromosome region 17q21, was originally thought to be a negative regulator of cell proliferation and a tumor suppressor. This anti-proliferative activity was later attributed to the 3' UTR of the PHB gene, and not to the actual protein. Mutations in human PHB have been linked to sporadic breast cancer. However, over-expression of PHB has been associated with a reduction in the androgen receptor activity and a reduction in PSA gene expression resulting in a decrease of androgen-dependent growth of cancerous prostate cells. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

Function

Prohibitins may have multiple functions including:

Mitochondrial function and morphology

Prohibitins are assembled into a ring-like structure with 16–20 alternating Phb1 and Phb2 subunits in the inner mitochondrial membrane. The precise molecular function of the PHB complex is not clear, but a role as chaperone for respiration chain proteins or as a general structuring scaffold required for optimal mitochondrial morphology and function are suspected. Recently, prohibitins have been demonstrated to be positive, rather than negative, regulators of cell proliferation in both plants and mice.

Transcriptional modulation

Both human prohibitins have also been suggested to be localized in the nucleus and modulate transcriptional activity by interacting with various transcription factors, including nuclear receptors, either directly or indirectly. However, little evidence for nuclear targeting and transcription factor-binding of prohibitins has been found in other organism (yeast, plants, C. elegans, etc.), indicating that this may be a specific function in mammalian cells.

Clinical significance

Human prohibitin 1 has some activity as a virus receptor protein, having been identified as a receptor for Chikungunya Virus (CHIKV) and Dengue Virus 2 (DENV-2). Little else is known about the activity of the prohibitins in viral pathogenesis.

Interactions

Prohibitin has been shown to interact with:

• ANXA2
• C-Raf,
• E2F1,
• HDAC1,
• P53,
• RB1,
• RBL1,
• RBL2,
• SMARCA2, and
• SMARCA4.

Drugs that bind to prohibitin

• Aurilide
• Fluorizoline
• Rocaglamide A

Prohibitinn in insect Prohibitin (PHB) is a highly conserved eukaryotic protein complex involved in multiple cellular processes. In insects, PHB has been identified as a potential target protein to insecticidal molecules acting as a receptor of PF2 insecticidal lectin in the midgut of Zabrotes subfasciatus larvae (bean pest) and Cry protein of Bacillus thuringiensis in Leptinotarsa decemlineata (Colorado potato beetle).

Further reading

• McClung JK, Jupe ER, Liu XT, Dell'Orco RT (1996). "Prohibitin: potential role in senescence, development, and tumor suppression". Experimental Gerontology. 30 (2): 99–124. doi:10.1016/0531-5565(94)00069-7. PMID 8591812. S2CID 9075146.
• Dell'Orco RT, McClung JK, Jupe ER, Liu XT (1996). "Prohibitin and the senescent phenotype". Experimental Gerontology. 31 (1–2): 245–52. doi:10.1016/0531-5565(95)02009-8. PMID 8706794. S2CID 22817314.
• Mishra S, Murphy LC, Nyomba BL, Murphy LJ (Apr 2005). "Prohibitin: a potential target for new therapeutics". Trends in Molecular Medicine. 11 (4): 192–7. doi:10.1016/j.molmed.2005.02.004. PMID 15823758.
• Rajalingam K, Rudel T (Nov 2005). "Ras-Raf signaling needs prohibitin". Cell Cycle. 4 (11): 1503–5. doi:10.4161/cc.4.11.2142. PMID 16294014.
• Sato T, Saito H, Swensen J, Olifant A, Wood C, Danner D, Sakamoto T, Takita K, Kasumi F, Miki Y (Mar 1992). "The human prohibitin gene located on chromosome 17q21 is mutated in sporadic breast cancer". Cancer Research. 52 (6): 1643–6. PMID 1540973.
• Dawson SJ, White LA (May 1992). "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin". The Journal of Infection. 24 (3): 317–20. doi:10.1016/S0163-4453(05)80037-4. PMID 1602151.
• White JJ, Ledbetter DH, Eddy RL, Shows TB, Stewart DA, Nuell MJ, Friedman V, Wood CM, Owens GA, McClung JK (Sep 1991). "Assignment of the human prohibitin gene (PHB) to chromosome 17 and identification of a DNA polymorphism". Genomics. 11 (1): 228–30. doi:10.1016/0888-7543(91)90126-Y. PMID 1684951.
• Altus MS, Wood CM, Stewart DA, Roskams AJ, Friedman V, Henderson T, Owens GA, Danner DB, Jupe ER, Dell'Orco RT (Jun 1995). "Regions of evolutionary conservation between the rat and human prohibitin-encoding genes". Gene. 158 (2): 291–4. doi:10.1016/0378-1119(95)00164-2. PMID 7607556.
• Ikonen E, Fiedler K, Parton RG, Simons K (Jan 1995). "Prohibitin, an antiproliferative protein, is localized to mitochondria". FEBS Letters. 358 (3): 273–7. doi:10.1016/0014-5793(94)01444-6. PMID 7843414. S2CID 31600675.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Sato T, Sakamoto T, Takita K, Saito H, Okui K, Nakamura Y (Sep 1993). "The human prohibitin (PHB) gene family and its somatic mutations in human tumors". Genomics. 17 (3): 762–4. doi:10.1006/geno.1993.1402. PMID 8244394.
• Jupe ER, Liu XT, Kiehlbauch JL, McClung JK, Dell'Orco RT (Apr 1996). "The 3' untranslated region of prohibitin and cellular immortalization". Experimental Cell Research. 224 (1): 128–35. doi:10.1006/excr.1996.0120. PMID 8612677.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Rasmussen RK, Ji H, Eddes JS, Moritz RL, Reid GE, Simpson RJ, Dorow DS (May 1998). "Two-dimensional electrophoretic analysis of mixed lineage kinase 2 N-terminal domain binding proteins". Electrophoresis. 19 (5): 809–17. doi:10.1002/elps.1150190535. PMID 9629920. S2CID 21204230.
• Wang S, Nath N, Adlam M, Chellappan S (Jun 1999). "Prohibitin, a potential tumor suppressor, interacts with RB and regulates E2F function". Oncogene. 18 (23): 3501–10. doi:10.1038/sj.onc.1202684. PMID 10376528.
• Wang S, Nath N, Fusaro G, Chellappan S (Nov 1999). "Rb and prohibitin target distinct regions of E2F1 for repression and respond to different upstream signals". Molecular and Cellular Biology. 19 (11): 7447–60. doi:10.1128/mcb.19.11.7447. PMC 84738. PMID 10523633.
• Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Villegas-Coronado D, Guzman-Partida AM, Aispuro-Hernandez E, Vazquez-Moreno L, Huerta-Ocampo JÁ, Sarabia-Sainz JA, et al. (2022). "Characterization and expression of prohibitin during the mexican bean weevil (Zabrotes subfasciatus, Boheman, 1833) larvae development". Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology. 262: 110770. doi:10.1016/j.cbpb.2022.110770. PMID 35644320. S2CID 249145357.
• Coates PJ, Nenutil R, McGregor A, Picksley SM, Crouch DH, Hall PA, Wright EG (May 2001). "Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence". Experimental Cell Research. 265 (2): 262–73. doi:10.1006/excr.2001.5166. PMID 11302691.
• Van Aken O, Pecenková T, van de Cotte B, De Rycke R, Eeckhout D, Fromm H, De Jaeger G, Witters E, Beemster GT, Inzé D, Van Breusegem F (Dec 2007). "Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development". The Plant Journal. 52 (5): 850–64. doi:10.1111/j.1365-313X.2007.03276.x. PMID 17883375.