Nuclear-inclusion-a endopeptidase (EC 3.4.22.44, potyvirus NIa protease) is a protease enzyme found in potyviruses.[1][2][3][4] This enzyme catalyses the following chemical reaction:
| Nuclear-inclusion-a endopeptidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.4.22.44 | ||||||||
| CAS no. | 139946-51-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- Hydrolyses glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus.
- e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln \ (Ser or Gly) for the enzyme from tobacco etch virus.
The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease).
References edit
- ^ Fellers, J.P.; Collins, G.B.; Hunt, A.G. (1998). "The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection". Crop Sci. 38: 1309–1319. doi:10.2135/cropsci1998.0011183x003800050030x.
- ^ Kim, D.-H.; Choi, K.Y. (1998). "Potyvirus NIa protease". In Barrett, A.J.; Rawlings, N.D.; Woessner, J.F. (eds.). Handbook of Proteolytic Enzymes. London: Academic Press. pp. 721–723.
- ^ Takahashi T, Nakanishi M, Yao Y, Uyeda I, Serizawa N (May 1998). "Direct formation of human interleukin-11 by cis-acting system of plant virus protease in Escherichia coli". Bioscience, Biotechnology, and Biochemistry. 62 (5): 953–8. doi:10.1271/bbb.62.953. PMID 9648226.
- ^ Kim DH, Hwang DC, Kang BH, Lew J, Han J, Song BD, Choi KY (December 1996). "Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity". Virology. 226 (2): 183–90. doi:10.1006/viro.1996.0645. PMID 8955037.
External links edit
- Nuclear-inclusion-a+endopeptidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
