First officer may refer to:
Transportation edit
- Chief mate, the second-in-command (usually) of a merchant ship
- First officer (aviation) or "co-pilot", the second pilot of a civil aircraft
Military edit
- Executive officer (military), the second-in-command of a naval vessel
- First officer, the most senior civil service position in the United States Foreign Service
- First officer, a rank in the Air Transport Auxiliary corresponding to Flight Lieutenant in the Royal Air Force
- First officer, a rank in the Women's Royal Naval Service corresponding to Lieutenant-Commander in the Royal Navy
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