DnaD is a 232 amino acid long protein that is part of the primosome involved in prokaryotic DNA replication.[1] In Bacillus subtilis, genetic analysis has revealed three primosomal proteins, DnaB, DnaD, and DnaI, that have no obvious homologues in E. coli. They are involved in primosome function both at arrested replication forks and at the chromosomal origin.[2]
| DNA replication protein DnaD | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | dnaD | ||||||
| Entrez | 939040 | ||||||
| RefSeq (Prot) | NP_390116.1 | ||||||
| UniProt | P39787 | ||||||
| Other data | |||||||
| Chromosome | genomic: 2.35 - 2.35 Mb | ||||||
| |||||||
DnaB and DnaD proteins are both multimeric and bind individually to DNA. DnaD induces DnaB to bind. DnaD alone and the DnaD/DnaB complex then interact with PriA of Bacillus subtilis at several DNA sites. This suggests that the nucleoprotein assembly is sequential in the PriA, DnaD, DnaB order.[3]
References edit
- ^ Bruand C, Sorokin A, Serror P, Ehrlich SD (February 1995). "Nucleotide sequence of the Bacillus subtilis dnaD gene". Microbiology. 141 ( Pt 2) (2): 321–2. doi:10.1099/13500872-141-2-321. PMID 7704260.
- ^ Ishigo-Oka D, Ogasawara N, Moriya S (March 2001). "DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication". Journal of Bacteriology. 183 (6): 2148–50. doi:10.1128/JB.183.6.2148-2150.2001. PMC 95117. PMID 11222620.
- ^ Marsin S, McGovern S, Ehrlich SD, Bruand C, Polard P (December 2001). "Early steps of Bacillus subtilis primosome assembly". The Journal of Biological Chemistry. 276 (49): 45818–25. doi:10.1074/jbc.M101996200. PMID 11585815.
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