Charles Mayo may refer to:
- Charles Horace Mayo (1865–1939), American physician
- Charles Herbert Mayo (1845–1929), English antiquarian
- Charles William Mayo (1898–1968), American surgeon
- Charles Mayo (cricketer) (1903–1943), Canadian-born English cricketer
- Charles Mayo (Anglo-Saxon scholar) (1767–1858), Rawlinson and Bosworth Professor of Anglo-Saxon at the University of Oxford
- Charles Mayo (golfer), English professional golfer
This disambiguation page lists articles about people with the same name.
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