Wikipedia

C10orf35

Chromosome 10 open reading frame 35 (c10orf35) is a gene that in humans, encodes for a protein-binding,[1] transmembrane[2] protein. The protein contains the domain of unknown function 4605 (DUF4605)[1] which belongs to the protein family pfam15378.[3] This gene is located at locus 10q22.1.[1]

The blue highlighted line shows the location of the c10orf35 gene on Chromosome 10.

General characteristics edit

The physical properties of the c10orf35 protein were analyzed, and the molecular weight was predicted to be 13.2 kdal,[4] and the isoelectric point was predicted to be 11.5.[4] The properties of DUF4605 were also analyzed, and the molecular weight was predicted to be 6.0 kdal.[4] The isoelectric point was predicted to be 6.9,[4] which is significantly less basic than the full protein.

Properties and structure edit

 
This image gives a predicted image of the main secondary structure features, conserved regions, and the post-translational modification site.

This protein includes a highly conserved region between amino acids 1 and 19 discovered by MSA.[5] DUF 4605 is located between amino acids 62 and 92.[1] The secondary structure of the c10orf35 protein was predicted to consist of a β-sheet between amino acids 3 and 5,[6] an α-helix between 61 and 72,[6] and a transmembrane α-helix between 92 and 112.[7] The serine in position 62 can be phosphorylated to form a phosphoserine group.[7]

Homology edit

Paralogs edit

The protein of interest has a similar, paralogous domain found in c4orf32,[8] which is conserved in mammals, birds, very few reptiles, and one amphibian.[9]

Orthologs edit

The protein coded for by c10orf35 has many orthologs found mainly in mammals, reptiles, few amphibians, one fish, and one invertebrate.[10] Based on a multiple sequence alignment between representative orthologs, DUF 4605 and a second, highly conserved region were confirmed.[5]

Clinical significance edit

Expression edit

 
This image shows the expression of the c10orf35 protein in various human tissues.[11]

The c10orf35 protein has expression over the 75th percentile in the brain, spinal cord, and male reproductive organs.[12] Within the male reproductive system, the RNA is found within the testis and prostate, while the protein is expressed in the epididymis.[13] In the brain, the RNA is found in the cerebral cortex, while the protein is expressed ubiquitously throughout the brain.[13]

Transcription regulation edit

Protein interactions edit

The c10orf35 protein has been experimentally shown by Affinity Capture-MS to interact with 7 other proteins[1] as shown below.

Interacting Protein Symbol Definition
ATP1B3 ATPase, Na+/K+ Transporting, Beta 3 Polypeptide
CD3E CD3e molecule, epsilon (CD3-TCR complex)
GABRE Gamma-aminobutyric acid (GABA) A receptor, epsilon
HTR3C 5-hydroxytryptamine (serotonin) receptor 3C, ionotropic
LPAR1 Lysophosphatidic acid receptor 1
SGCD Sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
ZACN Zinc activated ligand-gated ion channel

References edit

  1. ^ a b c d e "c10orf35". National Center For Biotechnology Information (NCBI) Gene.
  2. ^ "SOSUI". Classification and Secondary Structure Prediction of Membrane Proteins. Retrieved 6 May 2016.
  3. ^ "Conserved Protein Domain Family DUF4605". National Center For Biotechnology Information (NCBI) Structure. Retrieved 6 May 2016.
  4. ^ a b c d "Compute pI/Mw". ExPASy Bioinformatics Research Portal. Retrieved 6 May 2016.
  5. ^ a b "CLUSTALW Alignment Tool". San Diego Supercomputer Center (SDSC). Retrieved 6 May 2016.
  6. ^ a b "A Protein Secondary Structure Prediction Server". JPred. Retrieved 9 May 2016.
  7. ^ a b "c10orf35". UniProt. Retrieved 9 May 2016.
  8. ^ "c4orf32". National Center For Biotechnology Information (NCBI) Gene. Retrieved 6 May 2016.
  9. ^ "Standard Nucleotide BLASTN queried for c4orf32". National Center For Biotechnology Information (NCBI) Basic Local Alignment Searth Tool (BLAST). Retrieved 6 May 2016.
  10. ^ "Standard Nucleotide BLAST queried for c10orf35". National Center For Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST). Retrieved 6 May 2016.
  11. ^ "Gene Expression Omnibus (GEO) Profile For GDS3834". National Center For Biotechnology Information (NCBI). Retrieved 9 May 2016.
  12. ^ "Gene Expression Omnibus (GEO) Profile". National Center For Biotechnology Information. Retrieved 6 May 2016.
  13. ^ a b "c10orf35". The Human Protein Atlas. Retrieved 6 May 2016.
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