Aminopyrimidine aminohydrolase (EC 3.5.99.2, thiaminase, thiaminase II, tenA (gene)) is an enzyme with systematic name 4-amino-5-aminomethyl-2-methylpyrimidine aminohydrolase.[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction
| Aminopyrimidine aminohydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.5.99.2 | ||||||||
| CAS no. | 9024-80-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (1) 4-amino-5-aminomethyl-2-methylpyrimidine + H2O 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia
- (2) thiamine + H2O 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
This enzyme was previously known as thiaminase II.
References edit
- ^ Fujita A, Nose Y (July 1954). "The second type of bacterial thiaminase". The Journal of Vitaminology. 1 (1): 1–7. doi:10.5925/jnsv1954.1.1. PMID 13243520.
- ^ Ikehata H (1960). "Purification of thiaminase II". J. Gen. Appl. Microbiol. 6: 30–39. doi:10.2323/jgam.6.30.
- ^ Toms AV, Haas AL, Park JH, Begley TP, Ealick SE (February 2005). "Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II". Biochemistry. 44 (7): 2319–29. doi:10.1021/bi0478648. PMID 15709744.
- ^ Benach J, Edstrom WC, Lee I, Das K, Cooper B, Xiao R, Liu J, Rost B, Acton TB, Montelione GT, Hunt JF (May 2005). "The 2.35 A structure of the TenA homolog from Pyrococcus furiosus supports an enzymatic function in thiamine metabolism". Acta Crystallographica Section D. 61 (Pt 5): 589–98. doi:10.1107/S0907444905005147. PMID 15858269.
- ^ Jenkins AH, Schyns G, Potot S, Sun G, Begley TP (August 2007). "A new thiamin salvage pathway". Nature Chemical Biology. 3 (8): 492–7. doi:10.1038/nchembio.2007.13. PMID 17618314.
- ^ Jenkins AL, Zhang Y, Ealick SE, Begley TP (February 2008). "Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis". Bioorganic Chemistry. 36 (1): 29–32. doi:10.1016/j.bioorg.2007.10.005. PMC 2293645. PMID 18054064.
- ^ French JB, Begley TP, Ealick SE (September 2011). "Structure of trifunctional THI20 from yeast". Acta Crystallographica Section D. 67 (Pt 9): 784–91. doi:10.1107/S0907444911024814. PMC 3169313. PMID 21904031.
External links edit
- Aminopyrimidine+aminohydrolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
