The ZebraBox[1] is an automated analysis chamber for the non-intrusive video observation of different types of freshwater marine indicator species, such as Dania rerio and Pimephales promelas. It is a type of Larval Photomoter Response (LPR) assay, which is used to monitor the swimming behaviour of larvae.[2]
The ZebraBox contains a controlled enclosed system of 96-well plates containing a high-resolution camera fitted with an infrared light and a fixed-angle lens.[3] The lighting conditions and illumination patterns can be manually controlled for fish acclimation and the simulation of circadian rhythms.[4] The apparatus allows for the analysis of zebrafish locomotion and activity, thus it is used in the fields of drug discovery and toxicological studies.[5][6]
References edit
- ^ "ZebraBox". ViewPoint Life Sciences.
- ^ Dasgupta S, Simonich MT, Tanguay RL (2022). "Zebrafish Behavioral Assays in Toxicology". In Zhu H, Xia M (eds.). High-Throughput Screening Assays in Toxicology. Methods in Molecular Biology. Vol. 2474. New York, NY: Springer US. pp. 109–122. doi:10.1007/978-1-0716-2213-1_11. ISBN 978-1-0716-2213-1. PMID 35294760.
- ^ Lee DA, Oikonomou G, Prober DA (February 2022). "Large-scale Analysis of Sleep in Zebrafish". Bio-Protocol. 12 (3): e4313. doi:10.21769/BioProtoc.4313. PMC 8855086. PMID 35284597.
- ^ Wang W, Ma Q, Ding X, Xu Y, He M, Xu J, et al. (March 2022). "Developmental toxicity of bromoacetamide via the thyroid hormone receptors-mediated disruption of thyroid hormone homeostasis in zebrafish embryos". Ecotoxicology and Environmental Safety. 233: 113334. doi:10.1016/j.ecoenv.2022.113334. PMID 35203007. S2CID 247090575.
- ^ Nasri A, Lafon PA, Mezni A, Clair P, Cubedo N, Mahmoudi E, et al. (April 2022). "Developmental exposure to the A6-pesticide causes changes in tyrosine hydroxylase gene expression, neurochemistry, and locomotors behavior in larval zebrafish". Toxicology Mechanisms and Methods. 32 (8): 569–579. doi:10.1080/15376516.2022.2056100. PMID 35313786. S2CID 247599353.
- ^ Liu J, Xu Y, Liao G, Tu H, Huang Y, Peng T, et al. (February 2022). "The role of ambra1 in Pb-induced developmental neurotoxicity in zebrafish". Biochemical and Biophysical Research Communications. 594: 139–145. doi:10.1016/j.bbrc.2021.12.084. PMID 35085890. S2CID 245496902.