In enzymology, a taxadiene 5alpha-hydroxylase (EC 1.14.99.37) is an enzyme that catalyzes the chemical reaction
| taxadiene 5-alpha-hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.99.37 | ||||||||
| CAS no. | 9035-51-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
- taxa-4,11-diene + AH2 + O2 taxa-4(20),11-dien-5alpha-ol + A + H2O
The 3 substrates of this enzyme are taxa-4,11-diene, an electron acceptor AH2, and O2, whereas its 3 products are taxa-4(20),11-dien-5alpha-ol, the reduction product A, and H2O.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derive from O miscellaneous. The systematic name of this enzyme class is taxa-4,11-diene,hydrogen-donor:oxygen oxidoreductase (5alpha-hydroxylating). This enzyme participates in diterpenoid biosynthesis.
References edit
Further reading edit
- Hefner J, Rubenstein SM, Ketchum RE, Gibson DM, Williams RM, Croteau R (June 1996). "Cytochrome P450-catalyzed hydroxylation of taxa-4(5),11(12)-diene to taxa-4(20),11(12)-dien-5alpha-ol: the first oxygenation step in taxol biosynthesis". Chemistry & Biology. 3 (6): 479–89. doi:10.1016/S1074-5521(96)90096-4. PMID 8807878.
