Talk:Reporter gene

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GFP Fluorescence Edit edit

Latest comment: 1 month ago1 comment1 person in discussion

I made an edit for scientific accuracy because green fluorescent protein (GFP) glows green under blue light as well as under ultraviolet light.

This is supported by the article that is cited which says "under illumination with the 360–400 nm spectrum of light that excites GFP fluorescence"^[1] which corresponds to ultraviolet light.^[2]

I don't believe that my edit would constitute a unit conversion—or at least, not one that is valid under WP:SYNTH—hence why I provided a citation. Overall, the citations for that line could be tidied up, but I think the best fix would be a single source that encompasses both the primary source and covers the range of light.

Kilvin the Futz-y Enterovirus (talk) 20:43, 18 March 2024 (UTC)Reply

References

^ "Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells". The FASEB Journal. March 2005. doi:10.1096/fj.04-3180fje. ISSN 0892-6638. PMC 1242169. PMID 15640280.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ "Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222". Nature Structural Biology. Jan 2002. doi:10.1038/nsb739. ISSN 1072-8368. PMID 11740505.

Spamming Wikipedia edit

Latest comment: 16 years ago1 comment1 person in discussion

Biologicalworld.com has spammed wikipedia like no tomorrow. It is a site of only a few pages and a LOT of adsense. Not much information is given except for "protocols" which are not referenced, and cannot be trusted from a site of that quality.

check: Links from Wikipedia

The following have been cleaned up:

en.wikipedia.org/wiki/Plasmid
en.wikipedia.org/wiki/Gel_electrophoresis
en.wikipedia.org/wiki/Green_fluorescent_protein
en.wikipedia.org/wiki/Homology_(biology)
en.wikipedia.org/wiki/HeLa
en.wikipedia.org/wiki/Protease
en.wikipedia.org/wiki/Restriction_enzyme
en.wikipedia.org/wiki/Petri_dish
en.wikipedia.org/wiki/Structural_domain
en.wikipedia.org/wiki/Trypsin
en.wikipedia.org/wiki/Oligonucleotide
en.wikipedia.org/wiki/Transmission_electron_microscope
en.wikipedia.org/wiki/Agar_plate
en.wikipedia.org/wiki/Calcium_phosphate
en.wikipedia.org/wiki/Disulfide_bond
en.wikipedia.org/wiki/Denaturation_(biochemistry)
en.wikipedia.org/wiki/DNA_ligase
en.wikipedia.org/wiki/Wild_type
en.wikipedia.org/wiki/Tissue_culture
en.wikipedia.org/wiki/Transmission_electron_microscopy
en.wikipedia.org/wiki/Reporter_gene
en.wikipedia.org/wiki/Northern_blot
en.wikipedia.org/wiki/Protein_engineering
en.wikipedia.org/wiki/Sticky_end/blunt_end
en.wikipedia.org/wiki/Taq_polymerase
en.wikipedia.org/wiki/Protein_domain
en.wikipedia.org/wiki/Coomassie
en.wikipedia.org/wiki/Native_state
en.wikipedia.org/wiki/Chinese_Hamster_Ovary_cell
en.wikipedia.org/wiki/Peptidase
en.wikipedia.org/wiki/Visking_tubing
en.wikipedia.org/wiki/Streptavidin
en.wikipedia.org/wiki/Microtiter_plate
en.wikipedia.org/wiki/Subcloning
en.wikipedia.org/wiki/Ion_exchange_chromatography
en.wikipedia.org/wiki/Thermal_cycler
en.wikipedia.org/wiki/Bovine_serum_albumin
en.wikipedia.org/wiki/Phosphate_buffered_saline
en.wikipedia.org/wiki/Glutathione_S-transferase
en.wikipedia.org/wiki/HEPES
en.wikipedia.org/wiki/Ortholog
en.wikipedia.org/wiki/Proteases
en.wikipedia.org/wiki/Salting_out
en.wikipedia.org/wiki/Fetal_bovine_serum
en.wikipedia.org/wiki/Proteolytic_enzyme
en.wikipedia.org/wiki/DNA_end
en.wikipedia.org/wiki/Supernatant
en.wikipedia.org/wiki/ABTS
en.wikipedia.org/wiki/Conserved_sequence
en.wikipedia.org/wiki/Peptide_mass_fingerprinting
en.wikipedia.org/wiki/Dithiothreitol
en.wikipedia.org/wiki/Uranyl_acetate

and many more Sciencetalks (talk) 03:24, 4 January 2008 (UTC)Reply

References edit

Add topic

[1] "Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells". The FASEB Journal. March 2005. doi:10.1096/fj.04-3180fje. ISSN 0892-6638. PMC 1242169. PMID 15640280.{{cite journal}}: CS1 maint: unflagged free DOI (link)

[2] "Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222". Nature Structural Biology. Jan 2002. doi:10.1038/nsb739. ISSN 1072-8368. PMID 11740505.

[1]

[2]