Oogenesis

Oogenesis, ovogenesis, or oögenesis /ˌ.əˈɛnɪsɪs/[1] is the differentiation of the ovum (egg cell) into a cell competent to further develop when fertilized.[2] It is developed from the primary oocyte by maturation. Oogenesis is initiated in the embryonic stage.

Oogenesis is the process of the production of egg cells that takes places in the ovaries of Females
Oogenesis
Identifiers
MeSHD009866
TEE1.0.2.2.0.0.2
Anatomical terminology

Oogenesis in non-human mammalsEdit

 
Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum. (In mammals, the first polar body normally disintegrates before dividing, so only two polar bodies are produced.[citation needed])

In mammals, the first part of oogenesis starts in the germinal epithelium, which gives rise to the development of ovarian follicles, the functional unit of the ovary.

Oogenesis consists of several sub-processes: oocytogenesis, ootidogenesis, and finally maturation to form an ovum (oogenesis proper). Folliculogenesis is a separate sub-process that accompanies and supports all three oogenetic sub-processes.

Cell type ploidy/chromosomes chromatids Process Time of completion
Oogonium diploid/46(2N) 2C Oocytogenesis (mitosis) Third trimester
primary oocyte diploid/46(2N) 4C Ootidogenesis (meiosis I) (Folliculogenesis) Dictyate in prophase I for up to 50 years
secondary oocyte haploid/23(1N) 2C Ootidogenesis (meiosis II) Halted in metaphase II until fertilization
Ootid haploid/23(1N) 1C Ootidogenesis (meiosis II) Minutes after fertilization
Ovum haploid/23(1N) 1C

Oogonium —(Oocytogenesis)—> Primary Oocyte —(Meiosis I)—> First Polar body (Discarded afterward) + Secondary oocyte —(Meiosis II)—> Second Polar Body (Discarded afterward) + Ovum

Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle-coordinating centrosomes.[3][4]

The creation of oogoniaEdit

The creation of oogonia traditionally doesn't belong to oogenesis proper, but, instead, to the common process of gametogenesis, which, in the female human, begins with the processes of folliculogenesis, oocytogenesis, and ootidogenesis. Oogonia enter meiosis during embryonic development, becoming oocytes. Meiosis begins with DNA replication and meiotic crossing over. It then stops in early prophase.

Maintenance of meiotic arrestEdit

Mammalian oocytes are maintained in meiotic prophase arrest for a very long time -- months in mice, years in humans. Initially the arrest is due to lack of sufficient cell cycle proteins to allow meiotic progression. However, as the oocyte grows, these proteins are synthesized, and meiotic arrest becomes dependent on cyclic AMP.[5] The cyclic AMP is generated by the oocyte by adenylyl cyclase in the oocyte membrane. The adenylyl cyclase is kept active by a constitutively active G-protein-coupled receptor known as GPR3 and a G-protein, Gs, also present in the oocyte membrane.[6]

Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. Removal of the oocyte from the follicle causes meiosis to progress in the oocyte.[7] The cells that comprise the follicle, known as granulosa cells, are connected to each other by proteins known as gap junctions, that allow small molecules to pass between the cells. The granulosa cells produce a small molecule, cyclic GMP, that diffuses into the oocyte through the gap junctions. In the oocyte, cyclic GMP prevents the breakdown of cyclic AMP by the phosphodiesterase PDE3, and thus maintains meiotic arrest.[8] The cyclic GMP is produced by the guanylyl cyclase NPR2.[9]

Reinitiation of meiosis and stimulation of ovulation by luteinizing hormoneEdit

As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. Luteinizing hormone acts on receptors in the outer layers of granulosa cells of the follicle, causing a decrease in cyclic GMP in the granulosa cells.[10] Because the granulosa cells and oocyte are connected by gap junctions, cyclic GMP also decreases in the oocyte, causing meiosis to resume.[11] Meiosis then proceeds to second metaphase, where it pauses again until fertilization. Luteinizing hormone also stimulates gene expression leading to ovulation.[12]

 
Oogenesis in Eukaryotic Cells.(A) oogonium where the mitotic division occurs (B) differentiation and meiosis I begins (C) primary oocyte (D) meiosis I is completed and meiosis II begins (E) secondary oocyte (F) first polar body (G) ovulation must occur and the presence of the sperm penetration (fertilization) induces meiosis II to completion (H) ovum (I) second polar body

Human oogenesisEdit

 
Oogenesis throughout a women's life

OogenesisEdit

Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes, a process called oocytogenesis.[13] Oocytogenesis is complete either before or shortly after birth.

Number of primary oocytesEdit

It is commonly believed that, when oocytogenesis is complete, no additional primary oocytes are created, in contrast to the male process of spermatogenesis, where gametocytes are continuously created. In other words, primary oocytes reach their maximum development at ~20[14] weeks of gestational age, when approximately seven million primary oocytes have been created; however, at birth, this number has already been reduced to approximately 1-2 million.

Two publications have challenged the belief that a finite number of oocytes are set around the time of birth.[15][16] The renewal of ovarian follicles from germline stem cells (originating from bone marrow and peripheral blood) has been reported in the postnatal mouse ovary. In contrast, DNA clock measurements do not indicate ongoing oogenesis during human females' lifetimes.[17] Thus, further experiments are required to determine the true dynamics of small follicle formation.

OotidogenesisEdit

The succeeding phase of ootidogenesis occurs when the primary oocyte develops into an ootid. This is achieved by the process of meiosis. In fact, a primary oocyte is, by its biological definition, a cell whose primary function is to divide by the process of meiosis.[18]

However, although this process begins at prenatal age, it stops at prophase I. In late fetal life, all oocytes, still primary oocytes, have halted at this stage of development, called the dictyate. After menarche, these cells then continue to develop, although only a few do so every menstrual cycle.

Meiosis IEdit

Meiosis I of ootidogenesis begins during embryonic development, but halts in the diplotene stage of prophase I until puberty. The mouse oocyte in the dictyate (prolonged diplotene) stage actively repairs DNA damage, whereas DNA repair is not detectable in the pre-dictyate (leptotene, zygotene and pachytene) stages of meiosis.[19] For those primary oocytes that continue to develop in each menstrual cycle, however, synapsis occurs and tetrads form, enabling chromosomal crossover to occur. As a result of meiosis I, the primary oocyte has now developed into the secondary oocyte and the first polar body.

Meiosis IIEdit

Immediately after meiosis I, the haploid secondary oocyte initiates meiosis II. However, this process is also halted at the metaphase II stage until fertilization, if such should ever occur. If the egg is not fertilized, it is disintegrated and released (menstruation) and the secondary oocyte does not complete meiosis II (and doesn't become an ovum). When meiosis II has completed, an ootid and another polar body have now been created. The polar body is small in size.

FolliculogenesisEdit

Synchronously with ootidogenesis, the ovarian follicle surrounding the ootid has developed from a primordial follicle to a preovulatory one.

Maturation into ovumEdit

Both polar bodies disintegrate at the end of Meiosis II, leaving only the ootid, which then eventually undergoes maturation into a mature ovum.

The function of forming polar bodies is to discard the extra haploid sets of chromosomes that have resulted as a consequence of meiosis.

In vitro maturationEdit

In vitro maturation (IVM) is the technique of letting ovarian follicles mature in vitro. It can potentially be performed before an IVF. In such cases, ovarian hyperstimulation isn't essential. Rather, oocytes can mature outside the body prior to IVF. Hence, no (or at least a lower dose of) gonadotropins have to be injected in the body.[20] Immature eggs have been grown until maturation in vitro at a 10% survival rate, but the technique is not yet clinically available.[21] With this technique, cryopreserved ovarian tissue could possibly be used to make oocytes that can directly undergo in vitro fertilization.[21]

In vitro oogenesisEdit

By definition it means, to recapitulate mammalian oogenesis and producing fertilizable oocytes in vitro.it is a complex process involving several different cell types, precise follicular cell-oocyte reciprocal interactions, a variety of nutrients and combinations of cytokines, and precise growth factors and hormones depending on the developmental stage. [22]In 2016, two papers published by Morohaku et al. and Hikabe et al. reported in vitro procedures that appear to reproduce efficiently these conditions allowing for the production, completely in a dish, of a relatively large number of oocytes that are fertilizable and capable of giving rise to viable offspring in the mouse. This technique can be mainly benefited in cancer patients where in today's condition their ovarian tissue is cryopreserved for fertility preservation.Alternately to the autologous transplantation, the development of culture systems that support oocyte development from the primordial follicle stage represent a valid strategy to restore fertility. Over time, many studies have been conducted with the aim to optimize the characteristics of ovarian tissue culture systems and to better support the three main phases: 1) activation of primordial follicles; 2) isolation and culture of growing preantral follicles; 3) removal from the follicle environment and maturation of oocyte cumulus complexes. While complete oocyte in vitro development has been achieved in mouse, with the production of live offspring, the goal of obtaining oocytes of sufficient quality to support embryo development has not been completely reached into higher mammals despite decades of effort.[23]

Ovarian agingEdit

BRCA1 and ATM proteins are employed in repair of DNA double-strand break during meiosis. These proteins appear to have a critical role in resisting ovarian aging.[24] However, homologous recombinational repair of DNA double-strand breaks mediated by BRCA1 and ATM weakens with age in oocytes of humans and other species.[24] Women with BRCA1 mutations have lower ovarian reserves and experience earlier menopause than women without these mutations. Even in woman without specific BRCA1 mutations, ovarian aging is associated with depletion of ovarian reserves leading to menopause, but at a slower rate than in those with such mutations. Since older premenopausal women ordinarily have normal progeny, their capability for meiotic recombinational repair appears to be sufficient to prevent deterioration of their germline despite the reduction in ovarian reserve. DNA damages may arise in the germline during the decades long period in humans between early oocytogenesis and the stage of meiosis in which homologous chromosomes are effectively paired (dictyate stage). It has been suggested that such DNA damages may be removed, in large part, by mechanisms dependent on chromosome pairing, such as homologous recombination.[25]

Oogenesis in non-mammalsEdit

 
Diagram of oogenesis in a digenean (Platyhelminthes)

Some algae and the oomycetes produce eggs in oogonia. In the brown alga Fucus, all four egg cells survive oogenesis, which is an exception to the rule that generally only one product of female meiosis survives to maturity.

In plants, oogenesis occurs inside the female gametophyte via mitosis. In many plants such as bryophytes, ferns, and gymnosperms, egg cells are formed in archegonia. In flowering plants, the female gametophyte has been reduced to an eight-celled embryo sac within the ovule inside the ovary of the flower. Oogenesis occurs within the embryo sac and leads to the formation of a single egg cell per ovule.

In ascaris, the oocyte does not even begin meiosis until the sperm touches it, in contrast to mammals, where meiosis is completed in the estrus cycle.

In female Drosophila flies, genetic recombination occurs during meiosis. This recombination is associated with formation of DNA double-strand breaks and the repair of these breaks. [26] The repair process leads to crossover recombinants as well as at least three times as many noncrossover recombinants (e.g. arising by gene conversion without crossover).[26]

See alsoEdit

ReferencesEdit

Cho WK, Stern S, Biggers JD. 1974. Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro. J Exp Zool.187:383-386

  1. ^ Merriam-Webster Online Dictionary Definition: Oogenesis
  2. ^ Gilbert, Scott F. (2000-01-01). "Oogenesis". Cite journal requires |journal= (help)
  3. ^ Szollosi D, Calarco P, Donahue RP (1972). "Absence of centrioles in the first and second meiotic spindles of mouse oocytes". J Cell Sci. 11 (2): 521–541. PMID 5076360.
  4. ^ Manandhar G, Schatten H, Sutovsky P (January 2005). "Centrosome reduction during gametogenesis and its significance". Biol. Reprod. 72 (1): 2–13. doi:10.1095/biolreprod.104.031245. PMID 15385423. S2CID 37305534.
  5. ^ Jaffe LA, Egbert JR. 2017. Regulation of Mammalian Oocyte Meiosis by Intercellular Communication Within the Ovarian Follicle. Ann. Rev. Physiol. 79:237-260.
  6. ^ Mehlmann LM, Saeki Y, Tanaka S, Brennan TJ, Evsikov AV, Pendola FL, Knowles BB, Eppig JJ, Jaffe LA. 2004. The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes. Science 306:1947-1950
  7. ^ Edwards RG. 1965. Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature 20:349-351.
  8. ^ Norris RP, Ratzan WJ, Freudzon M, Mehlmann LM, Krall J, Movsesian MA, Wang H, Ke H, Nikolaev VO, Jaffe LA. 2009. Cyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte. Development 136:1869-1878.
  9. ^ Zhang M, Su YQ, Sugiura K, Xia G, Eppig JJ. 2010. Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes. Science 330:366-369
  10. ^ Jaffe LA, Egbert JR. 2017. Regulation of Mammalian Oocyte Meiosis by Intercellular Communication Within the Ovarian Follicle. Ann. Rev. Physiol. 79:237-260.
  11. ^ Shuhaibar LC, Egbert JR, Norris RP, Lampe PD, Nikolaev VO, Thunemann M, Wen L, Feil R, Jaffe LA Intercellular signaling via cyclic GMP diffusion through gap junctions restarts meiosis in mouse ovarian follicles. Proc. Natl. Acad. Sci. USA 112: 5527-5532.
  12. ^ Richards JS, Ascoli M. 2018. Endocrine, paracrine, and autocrine signaling pathways that regulate ovulation. Trends Endocrinol. Metab. 29:313-325.
  13. ^ NCBI - The saga of the germ line
  14. ^ Lobo RA (September 2003). "Early ovarian ageing: a hypothesis. What is early ovarian ageing?". Hum. Reprod. 18 (9): 1762–4. doi:10.1093/humrep/deg377. PMID 12923124.
  15. ^ Johnson J, Bagley J, Skaznik-Wikiel M, et al. (July 2005). "Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood". Cell. 122 (2): 303–15. doi:10.1016/j.cell.2005.06.031. PMID 16051153. S2CID 19006732.
  16. ^ Johnson J, Canning J, Kaneko T, Pru J, Tilly J (2004). "Germline stem cells and follicular renewal in the postnatal mammalian ovary". Nature. 428 (6979): 145–50. doi:10.1038/nature02316. PMID 15014492. S2CID 1124530.
  17. ^ Forster P, Hohoff C, Dunkelmann B, Schürenkamp M, Pfeiffer H, Neuhuber F, Brinkmann B (2015). "Elevated germline mutation rate in teenage fathers". Proc R Soc B. 282 (1803): 20142898. doi:10.1098/rspb.2014.2898. PMC 4345458. PMID 25694621.
  18. ^ Biochem
  19. ^ Guli CL, Smyth DR (1988). "UV-induced DNA repair is not detectable in pre-dictyate oocytes of the mouse". Mutat Res. 208 (2): 115–119. doi:10.1016/s0165-7992(98)90010-0. PMID 3380109.
  20. ^ "Vejledning om kunstig befrugtning 2006 (Danish)" (PDF). Archived from the original (PDF) on 2012-03-09. Retrieved 2011-01-29.
  21. ^ a b
  22. ^ Jun-Jie Wang 1, Wei Ge 1, Jing-Cai Liu 1, Francesca Gioia Klinger 2, Paul W Dyce 3, Massimo De Felici 2, Wei Shen 1 (2017). "Complete in vitro oogenesis: retrospects and prospects". Cell Death Differ. 24 (11): 1845–1852. doi:10.1038/cdd.2017.134. PMC 5635224. PMID 28841213.CS1 maint: multiple names: authors list (link)
  23. ^ Raffaella Fabbri, Chiara Zamboni, Rossella Vicenti, Maria Macciocca, Roberto Paradisi, Renato Seracchioli (2018). "Update on oogenesis in vitro". Minerva Ginecol. 70 (5): 588–608. doi:10.23736/S0026-4784.18.04273-9. PMID 29999288.CS1 maint: multiple names: authors list (link)
  24. ^ a b Turan V, Oktay K. BRCA-related ATM-mediated DNA double-strand break repair and ovarian aging. Hum Reprod Update. 2020 Jan 1;26(1):43-57. doi: 10.1093/humupd/dmz043. PMID: 31822904; PMCID: PMC6935693
  25. ^ Bernstein C. Why are babies young? Meiosis may prevent aging of the germ line. Perspect Biol Med. 1979 Summer;22(4):539-44. doi: 10.1353/pbm.1979.0041. PMID: 573881
  26. ^ a b Mehrotra S, McKim KS. Temporal analysis of meiotic DNA double-strand break formation and repair in Drosophila females. PLoS Genet. 2006 Nov 24;2(11):e200. PMID:17166055
Bibliography
  • Manandhar G, Schatten H and Sutovsky P (2005). Centrosome reduction during gametogenesis and its significance. Biol Reprod, 72(1)2-13.

External linksEdit