Artificial insemination (AI) is the deliberate introduction of sperm into a female's cervix or uterine cavity for the purpose of achieving a pregnancy through in vivo fertilization by means other than sexual intercourse. It is a fertility treatment for humans, and is common practice in animal breeding, including dairy cattle (see Frozen bovine semen) and pigs.
Schematic illustration of artificial insemination
Artificial insemination may employ assisted reproductive technology, sperm donation and animal husbandry techniques. Artificial insemination techniques available include intracervical insemination and intrauterine insemination. The beneficiaries of artificial insemination are women who desire to give birth to their own child who may be single, women who are in a lesbian relationship or women who are in a heterosexual relationship but with a male partner who is infertile or who has a physical impairment which prevents full intercourse from taking place. Intracervical insemination (ICI) is the easiest and most common insemination technique and can be used in the home for self-insemination without medical practitioner assistance. Compared with natural insemination (i.e., insemination by sexual intercourse), artificial insemination can be more expensive and more invasive, and may require professional assistance.
Some countries have laws which restrict and regulate who can donate sperm and who is able to receive artificial insemination, and the consequences of such insemination. Some women who live in a jurisdiction which does not permit artificial insemination in the circumstance in which she finds herself may travel to another jurisdiction which permits it.
- 1 In humans
- 2 In animals
- 3 See also
- 4 References
- 5 Further reading
- 6 External links
This section needs additional citations for verification. (September 2017) (Learn how and when to remove this template message)
The first reported case of artificial insemination by donor occurred in 1884: Dr. William H. Pancoast, a professor in Philadelphia, took sperm from his "best looking" student to inseminate an anesthetized woman. The case was reported 25 years later in a medical journal. The sperm bank was developed in Iowa starting in the 1920s in research conducted by University of Iowa medical school researchers Jerome Sherman and Raymond Bunge.
In the 1980s, direct intraperitoneal insemination (DIPI) was occasionally used, where doctors injected sperm into the lower abdomen through a surgical hole or incision, with the intention of letting them find the oocyte at the ovary or after entering the genital tract through the ostium of the fallopian tube.
The sperm used in artificial insemination may be provided by either the woman's husband or partner (partner sperm) or by a known or anonymous sperm donor (see sperm donation (donor sperm)).
If the procedure is successful, the woman will conceive and carry a baby to term in the normal manner. A pregnancy resulting from artificial insemination is no different from a pregnancy achieved by sexual intercourse. In all cases of artificial insemination, the recipient woman will be the biological mother of any child produced, and the male whose sperm is used will be the biological father.
There are multiple methods used to obtain the semen necessary for artificial insemination. Some methods require only men, while others require a combination of a male and female. Those that require only men to obtain semen are masturbation or the aspiration of sperm by means of a puncture of the testicle and epididymus. Methods of collecting semen that involve a combination of a male and female include interrupted intercourse, intercourse with a 'collection condom', or the post-coital aspiration of the semen from the vagina.
There are a number of reasons why a woman with a male partner would use artificial insemination to achieve pregnancy. For example, a woman's immune system may be rejecting her partner's sperm as invading molecules. Women who have issues with the cervix – such as cervical scarring, cervical blockage from endometriosis, or thick cervical mucus – may also benefit from artificial insemination, since the sperm must pass through the cervix to result in fertilization.
In the case of heterosexual couples who are finding it difficult to conceive, before artificial insemination is turned to as the solution, doctors will require an examination of both the male and female involved in order to remove any and all physical hindrances that are preventing them from naturally achieving a pregnancy. The couple is also given a fertility test to determine the motility, number, and viability of the male's sperm and the success of the female's ovulation. From these tests, the doctor may or may not recommend a form of artificial insemination.
Timing is critical, as the window and opportunity for fertilization is little more than twelve hours from the release of the ovum. To increase the chance of success, the woman's menstrual cycle is closely observed, often using ovulation kits, ultrasounds or blood tests, such as basal body temperature tests over, noting the color and texture of the vaginal mucus, and the softness of the nose of her cervix. To improve the success rate of AI, drugs to create a stimulated cycle may be used, but the use of such drugs also results in an increased chance of a multiple birth.
Sperm can be provided fresh or washed. The washing of sperm increases the chances of fertilization. Pre- and post-concentration of motile sperm is counted. Sperm from a sperm bank will be frozen and quarantined for a period, and the donor will be tested before and after production of the sample to ensure that he does not carry a transmissible disease. For fresh shipping, a semen extender is used.
If sperm is provided by a private donor, either directly or through a sperm agency, it is usually supplied fresh, not frozen, and it will not be quarantined. Donor sperm provided in this way may be given directly to the recipient woman or her partner, or it may be transported in specially insulated containers. Some donors have their own freezing apparatus to freeze and store their sperm.
Semen used is used either fresh, raw, or frozen. Where donor sperm is supplied by a sperm bank, it will always be quarantined and frozen, and will need to be thawed before use. The sperm is ideally donated after 2-3 days of abstinence, without lubrication as the lubricant can inhibit the sperm motility. When an ovum is released, semen is introduced into the woman's vagina, uterus or cervix, depending on the method being used.
Sperm is occasionally inserted twice within a 'treatment cycle'.
Intracervical insemination (ICI) simulates an ejaculation of semen by the penis into the vagina during intercourse. It is painless and is the simplest, easiest and most common method of artificial insemination. ICI involves the introduction of unwashed or raw semen into the vagina at the entrance to the cervix, usually by means of a needleless syringe.
ICI is commonly used in the home, by self-insemination and practitioner insemination. Raw semen from a private donor may be used for ICI. Semen supplied by a sperm bank prepared for ICI or IUI use is suitable for ICI.
During ICI, air is expelled from a needleless syringe which is then filled with semen which has been allowed to liquify. A specially-designed syringe, wider and with a more rounded end, may be used for this purpose. Any further enclosed air is removed by gently pressing the plunger forward. The woman lies on her back and the syringe is inserted into the vagina. Care is optimal when inserting the syringe, so that the tip is as close to the entrance to the cervix as possible. A vaginal speculum may be used for this purpose. The plunger is then slowly pushed forward and the semen in the syringe is gently emptied deep into the vagina. It is important that the syringe is emptied slowly for best results. The syringe may be left in place for several minutes before removal. The woman can bring herself to orgasm so that the cervix 'dips down' into the pool of semen, again replicating closely vaginal intercourse, and this may improve the success rate. The woman is advised to lie still for about half-an-hour to improve the success rate.
One insemination during a cycle is usually sufficient. Additional inseminations may not improve the chances of a pregnancy.
Ordinary sexual lubricants should not be used in the process, but special fertility or 'sperm-friendly' lubricants can be used for increased ease and comfort.
When performed at home without the presence of a professional, aiming the sperm in the vagina at the neck of the cervix may be more difficult to achieve and the effect may be to 'flood' the vagina with semen, rather than to target it specifically at the entrance to the cervix. This procedure is therefore sometimes referred to as intravaginal insemination (IVI). Sperm supplied by a sperm bank will be frozen and must be allowed to thaw before insemination. The sealed end of the straw itself must be cut off and the open end of the straw is usually fixed straight on to the tip of the syringe, allowing the contents to be drawn into the syringe. Sperm from more than one straw can generally be used in the same syringe. Where fresh semen is used, this must be allowed to liquefy before inserting it into the syringe, or alternatively, the syringe may be back-loaded.
A conception cap, which is a form of conception device, may be inserted into the vagina following insemination and may be left in place for several hours. Using this method, a woman may go about her usual activities while the cervical cap holds the semen in the vagina close to the entrance to the cervix. Advocates of this method claim that it increases the chances of conception. One advantage with the conception device is that fresh, non-liquefied semen may be used. The male may ejaculate straight into the cap so that his fresh semen can be inserted immediately into the vagina without waiting for it to liquefy, although a collection cup may also be used. Other methods may be used to insert semen into the vagina notably involving different uses of a conception cap. This may, for example, be inserted filled with sperm which does not have to be liquefied. Alternatively, a specially designed conception cap with a tube attached may be inserted empty into the vagina after which liquefied semen is poured into the tube. These methods are designed to ensure that semen is inseminated as close as possible to the cervix and that it is kept in place there to increase the chances of conception.
Intrauterine insemination (IUI) involves injection of washed sperm into the uterus with a catheter. If unwashed semen is used, it may elicit uterine cramping, expelling the semen and causing pain, due to content of prostaglandins. (Prostaglandins are also the compounds responsible for causing the myometrium to contract and expel the menses from the uterus, during menstruation.) Resting on the table for fifteen minutes after an IUI is optimal for the woman to increase the pregnancy rate.
The indications to perform an intrauterine insemination are usually a moderate male factor, the incapability to ejaculate in vagina and an idiopathic infertility.
Unlike ICI, intrauterine insemination normally requires a medical practitioner to perform the procedure. One of the requirements is to have at least one permeable tube, proved by hysterosalpingography. The infertility duration is also important. A female under 30 years of age has optimal chances with IUI; for the man, a TMS of more than 5 million per ml is optimal. In practice, donor sperm will satisfy these criteria. A promising cycle is one that offers two follicles measuring more than 16 mm, and estrogen of more than 500 pg/mL on the day of hCG administration. A short period of ejaculatory abstinence before intrauterine insemination is associated with higher pregnancy rates. However, GnRH agonist administration at the time of implantation does not improve pregnancy outcome in intrauterine insemination cycles according to a randomized controlled trial.
IUI is a more efficient method of artificial insemination than ICI and, because of its generally higher success rate, is usually the insemination procedure of choice for single women and lesbians using donor semen in a fertility centre and who are less likely to have fertility issues of their own. Enabling the sperm to be inserted directly into the womb will produce a better chance of conceiving.
It is also a method used by couples using donor sperm in a fertility centre.
The steps to follow in order to perform an intrauterine insemination are: Mild Controlled Ovarian Stimulation (COS), Ovulation Induction, Semen capacitation and Luteal Phase support.
IUI can be used in conjunction with controlled ovarian hyperstimulation (COH). Clomiphene Citrate is the first line, Letrozole is second line, in order to stimulate ovaries before moving on to IVF. Still, advanced maternal age causes decreased success rates; women aged 38–39 years appear to have reasonable success during the first two cycles of ovarian hyperstimulation and IUI. However, for women aged over 40 years, there appears to be no benefit after a single cycle of COH/IUI. Medical experts therefore recommend considering in vitro fertilization after one failed COH/IUI cycle for women aged over 40 years.
A double intrauterine insemination theoretically increases pregnancy rates by decreasing the risk of missing the fertile window during ovulation. However, a randomized trial of insemination after ovarian hyperstimulation found no difference in live birth rate between single and double intrauterine insemination.
Intrauterine tuboperitoneal inseminationEdit
Intrauterine tuboperitoneal insemination (IUTPI) involves injection of washed sperm into both the uterus and fallopian tubes. The cervix is then clamped to prevent leakage to the vagina, best achieved with a specially designed double nut bivalve (DNB) speculum. The sperm is mixed to create a volume of 10 ml, sufficient to fill the uterine cavity, pass through the interstitial part of the tubes and the ampulla, finally reaching the peritoneal cavity and the Pouch of Douglas where it would be mixed with the peritoneal and follicular fluid. IUTPI can be useful in unexplained infertility, mild or moderate male infertility, and mild or moderate endometriosis. In non-tubal sub fertility, fallopian tube sperm perfusion may be the preferred technique over intrauterine insemination.
Intratubal insemination (ITI) involves injection of washed sperm into the fallopian tube, although this procedure is no longer generally regarded as having any beneficial effect compared with IUI. ITI however, should not be confused with gamete intrafallopian transfer, where both eggs and sperm are mixed outside the woman's body and then immediately inserted into the fallopian tube where fertilization takes place.
The rate of successful pregnancy for artificial insemination are 10-15% per menstrual cycle using ICI, and 15–20% per cycle for IUI.[unreliable source?] In IUI, about 60 to 70% have achieved pregnancy after 6 cycles.
However, these pregnancy rates may be very misleading, since many factors have to be included to give a meaningful answer, e.g. definition of success and calculation of the total population. These rates can be influenced by age, overall reproductive health, and if the patient had an orgasm during the insemination. The literature is conflicting on immobilization after insemination has increasing the chances of pregnancy  Previous data suggests that it is statistically significant for the patient to remain immobile for 15 minutes after insemination, while other review article claims that it is not.  A point of consideration, is that it does cost the patient or healthcare system to remain immobile for 15 minutes if it does increase the chances. For couples with unexplained infertility, unstimulated IUI is no more effective than natural means of conception.
The pregnancy rate also depends on the total sperm count, or, more specifically, the total motile sperm count (TMSC), used in a cycle. The success rate increases with increasing TMSC, but only up to a certain count, when other factors become limiting to success. The summed pregnancy rate of two cycles using a TMSC of 5 million (may be a TSC of ~10 million on graph) in each cycle is substantially higher than one single cycle using a TMSC of 10 million. However, although more cost-efficient, using a lower TMSC also increases the average time taken to achieve pregnancy. Women whose age is becoming a major factor in fertility may not want to spend that extra time.
Samples per childEdit
The number of samples (ejaculates) required to give rise to a child varies substantially from person to person, as well as from clinic to clinic. However, the following equations generalize the main factors involved:
For intracervical insemination:
- N is how many children a single sample can give rise to.
- Vs is the volume of a sample (ejaculate), usually between 1.0 mL and 6.5 mL
- c is the concentration of motile sperm in a sample after freezing and thawing, approximately 5–20 million per ml but varies substantially
- rs is the pregnancy rate per cycle, between 10% to 35% 
- nr is the total motile sperm count recommended for vaginal insemination (VI) or intra-cervical insemination (ICI), approximately 20 million pr. ml.
The pregnancy rate increases with increasing number of motile sperm used, but only up to a certain degree, when other factors become limiting instead.
With these numbers, one sample would on average help giving rise to 0.1–0.6 children, that is, it actually takes on average 2–5 samples to make a child.
For intrauterine insemination, a centrifugation fraction (fc) may be added to the equation:
- fc is the fraction of the volume that remains after centrifugation of the sample, which may be about half (0.5) to a third (0.33).
On the other hand, only 5 million motile sperm may be needed per cycle with IUI (nr=5 million)
Thus, only 1–3 samples may be needed for a child if used for IUI.
One of the key issues arising from the rise of dependency on assisted reproductive technology (ARTs) is the pressure placed on couples to conceive; 'where children are highly desired, parenthood is culturally mandatory, and childlessness socially unacceptable'.
The medicalization of infertility creates a framework in which individuals are encouraged to think of infertility quite negatively. In many cultures donor insemination is religiously and culturally prohibited, often meaning that less accessible "high tech" and expensive ARTs, like IVF, are the only solution.
An over-reliance on reproductive technologies in dealing with infertility prevents many – especially, for example, in the "infertility belt" of central and southern Africa – from dealing with many of the key causes of infertility treatable by artificial insemination techniques; namely preventable infections, dietary and lifestyle influences.
If good records are not kept, the offspring when grown up risk accidental incest.
Some countries restrict artificial insemination in a variety of ways. For example, some countries do not permit AI for single women, and some Muslim countries do not permit the use of donor sperm. As of May 2013, the following European countries permit medically assisted AI for single women:
- United Kingdom
Reasons and techniquesEdit
It may be used for many reasons, including to allow a male to inseminate a much larger number of females, to allow use of genetic material from males separated by distance or time, to overcome physical breeding difficulties, to control the paternity of offspring, to synchronise births, to avoid injury incurred during natural mating, and to avoid the need to keep a male at all (such as for small numbers of females or in species whose fertile males may be difficult to manage).
Semen is collected, extended, then cooled or frozen. It can be used on site or shipped to the female's location. If frozen, the small plastic tube holding the semen is referred to as a straw. To allow the sperm to remain viable during the time before and after it is frozen, the semen is mixed with a solution containing glycerol or other cryoprotectants. An extender is a solution that allows the semen from a donor to impregnate more females by making insemination possible with fewer sperm. Antibiotics, such as streptomycin, are sometimes added to the sperm to control some bacterial venereal diseases. Before the actual insemination, estrus may be induced through the use of progestogen and another hormone (usually PMSG or Prostaglandin F2α).
The first viviparous animal to be artificially fertilized was a dog. The experiment was conducted with success by the Italian Lazzaro Spallanzani in 1780. Another pioneer was the Russian Ilya Ivanov in 1899. In 1935, diluted semen from Suffolk sheep was flown from Cambridge in Britain to Kraków, Poland, as part of an international research project. The participants included Prawochenki (Poland), Milovanoff (USSR), Hammond and Walton (UK), and Thomasset (Uruguay).
Modern artificial insemination was pioneered by John O. Almquist of Pennsylvania State University. He improved breeding efficiency by the use of antibiotics (first proven with penicillin in 1946) to control bacterial growth, decreasing embryonic mortality, and increase fertility. This, and various new techniques for processing, freezing, and thawing of frozen semen significantly enhanced the practical utilization of AI in the livestock industry, and earned him the 1981 Wolf Foundation Prize in Agriculture. Many techniques developed by him have since been applied to other species, including humans.
Artificial insemination is used in many non-human animals, including sheep, horses, cattle, pigs, dogs, pedigree animals generally, zoo animals, turkeys and creatures as tiny as honeybees and as massive as orcas (killer whales).
Artificial insemination of farm animals is common in the developed world, especially for breeding dairy cattle (75% of all inseminations). Swine are also bred using this method (up to 85% of all inseminations). It provides an economical means for a livestock breeder to improve their herds utilizing males having very desirable traits.
Although common with cattle and swine, AI is not as widely practised in the breeding of horses. A small number of equine associations in North America accept only horses that have been conceived by "natural cover" or "natural service" – the actual physical mating of a mare to a stallion – the Jockey Club being the most notable of these, as no AI is allowed in Thoroughbred breeding. Other registries such as the AQHA and warmblood registries allow registration of foals created through AI, and the process is widely used allowing the breeding of mares to stallions not resident at the same facility – or even in the same country – through the use of transported frozen or cooled semen.
In modern species conservation, semen collection and artificial insemination is used also in birds. In 2013 scientist of the Justus-Liebig-University of Giessen, Germany, from the working group of Michael Lierz, Clinic for birds, reptiles, amphibians and fish, developed a novel technique for semen collection and artificial insemination in parrots producing the world's first macaw by assisted reproduction.
Scientists working with captive orcas were able to pioneer the technique in the early 2000s, resulting in "the first successful conceptions, resulting in live offspring, using artificial insemination in any cetacean species". John Hargrove, a Seaworld trainer, describes Kasatka as being the first orca to receive AI.
|Wikimedia Commons has media related to Artificial insemination.|
- Seattle Sperm Bank
- Yuko, Elizabeth (2016-01-08). "The First Artificial Insemination Was an Ethical Nightmare". The Atlantic. Retrieved 2019-07-17.
- "Letter to the Editor: Artificial Impregnation". The Medical World: 163–164. April 1909. Archived from the original on 2012-07-24. (cited in Gregoire, A.; Mayer, R. (1964). "The impregnators". Fertility and Sterility. 16: 130–4. doi:10.1016/s0015-0282(16)35476-0. PMID 14256095.)
- Kara W. Swanson, “The Birth of the Sperm Bank,” Annals of Iowa, 71 (Summer 2012), 241–76.
- Cox, Lauren (3 February 2010). "Oral Sex, a Knife Fight and Then Sperm Still Impregnated Girl. Account of a Girl Impregnated After Oral Sex Shows Sperms' Incredible Survivability". abc NEWS.
- Cimino, C.; Guastella, G.; Comparetto, G.; Gullo, D.; Perino, A.; Benigno, M.; Barba, G.; Cittadini, E. (1988). "Direct intraperitoneal insemination (DIPI) for the treatment of refractory infertility unrelated to female organic pelvic disease". Acta Europaea Fertilitatis. 19 (2): 61–68. PMID 3223194.
- Robinson, Sarah (2010-06-24). "Professor". International Federation of Gynecology and Obstetrics. Archived from the original on 2012-11-04. Retrieved 2012-12-27.
- Adams, Robert (1988). in vitro fertilization technique. Monterey CA.
- Ginsburg, Elizabeth (June 4, 2018). "Procedure for intrauterine insemination (IUI) using processed sperm". Uptodate.com.
- European Sperm Bank USA
- Laurie Barclay. "Immobilization May Improve Pregnancy Rate After Intrauterine Insemination". Medscape Medical News. Retrieved October 31, 2009.
- Merviel P, Heraud MH, Grenier N, Lourdel E, Sanguinet P, Copin H (November 2008). "Predictive factors for pregnancy after intrauterine insemination (IUI): An analysis of 1038 cycles and a review of the literature". Fertil. Steril. 93 (1): 79–88. doi:10.1016/j.fertnstert.2008.09.058. PMID 18996517.
- Marshburn PB, Alanis M, Matthews ML, et al. (September 2009). "A short period of ejaculatory abstinence before intrauterine insemination is associated with higher pregnancy rates". Fertil. Steril. 93 (1): 286–8. doi:10.1016/j.fertnstert.2009.07.972. PMID 19732887.
- Bellver J, Labarta E, Bosch E, et al. (June 2009). "GnRH agonist administration at the time of implantation does not improve pregnancy outcome in intrauterine insemination cycles: a randomized controlled trial". Fertil. Steril. 94 (3): 1065–71. doi:10.1016/j.fertnstert.2009.04.044. PMID 19501354.
- Harris, I.; Missmer, S.; Hornstein, M. (2010). "Poor success of gonadotropin-induced controlled ovarian hyperstimulation and intrauterine insemination for older women". Fertility and Sterility. 94 (1): 144–148. doi:10.1016/j.fertnstert.2009.02.040. PMID 19394605.
- Bagis T, Haydardedeoglu B, Kilicdag EB, Cok T, Simsek E, Parlakgumus AH (May 2010). "Single versus double intrauterine insemination in multi-follicular ovarian hyperstimulation cycles: a randomized trial". Hum Reprod. 25 (7): 1684–90. doi:10.1093/humrep/deq112. PMID 20457669.
- Leonidas Mamas, M.D. (March 2006). "Comparison of fallopian tube sperm perfusion and intrauterine tuboperitoneal insemination:a prospective randomized study". Fertility and Sterility. 85 (3): 735–740. doi:10.1016/j.fertnstert.2005.08.025. PMID 16500346.
- G S Shekhawat, MD (2012). "Intrauterine insemination versus Fallopian tube sperm perfusion in non-tubal infertility". Internet Medical Journal. Archived from the original on 2014-05-02. Retrieved 2012-01-24.
- Hurd WW, Randolph JF, Ansbacher R, Menge AC, Ohl DA, Brown AN (February 1993). "Comparison of intracervical, intrauterine, and intratubal techniques for donor insemination". Fertil. Steril. 59 (2): 339–42. doi:10.1016/S0015-0282(16)55671-4. PMID 8425628.
- Utrecht CS News Subject: Infertility FAQ (part 4/4)
- Intrauterine insemination. Information notes from the fertility clinic at Aarhus University Hospital, Skejby. By PhD Ulrik Kesmodel et al.
- Cordary, D (December 2017). "Immobilization versus immediate mobilization after intrauterine insemination: A systematic review and meta-analysis". Journal of Gynecology Obstetrics and Human Reproduction. 46 (10): 747–751. doi:10.1016/j.jogoh.2017.09.005. PMID 28964965 – via Elsevier Science Direct.
- Mol, Ben Willem J.; Veen, Fulco van der; Janssen, Catharina A. H.; Mochtar, Monique H.; Gerards, Mariette H.; Dessel, Thierry J. H. M. Van; Cox, Tessa; Maas, Pettie; Flierman, Paul A. (2009-10-30). "Immobilisation versus immediate mobilisation after intrauterine insemination: randomised controlled trial". BMJ. 339: b4080. doi:10.1136/bmj.b4080. ISSN 0959-8138. PMC 2771078. PMID 19875843.
- "Fertility treatments 'no benefit'". BBC News. 7 August 2008.
- Bhattacharya S, Harrild K, Mollison J, et al. (2008). "Clomifene citrate or unstimulated intrauterine insemination compared with expectant management for unexplained infertility: pragmatic randomised controlled trial". BMJ. 337: a716. doi:10.1136/bmj.a716. PMC 2505091. PMID 18687718.
- Essig, Maria G. (2007-02-20). Van Houten, Susan; Landauer, Tracy (eds.). "Semen Analysis". Healthwise. Reviewed by Martin Gabica and Avery L. Seifert. WebMD. Retrieved 2007-08-05.
- Cryos International – What is the expected pregnancy rate (PR) using your donor semen? Archived January 7, 2011, at the Wayback Machine
- Cryos International – How much sperm should I order? Archived 2013-08-28 at the Wayback Machine
- Inhorn, Marcia C. (2003). "Global infertility and the globalization of new reproductive technologies: Illustrations from Egypt". Social Science and Medicine. 56 (9): 1837–51. doi:10.1016/s0277-9536(02)00208-3. PMID 12650724.
- Rainbow Europe Country Index[permanent dead link]
- Chet, Ilan (2009). "John O. Almquist". Wolf Prize in Agriculture. World Scientific. pp. 121–134. ISBN 978-981-283-585-7.
- "Equine Artificial Insemination". www.equine-reproduction.com. Retrieved 2018-03-01.
- The Jockey Club has never allowed artificial insemination. Archived September 20, 2008, at the Wayback Machine
- Lierz M, Reinschmidt M, Müller H, Wink M, Neumann D (2013). "A novel method for semen collection and artificial insemination in large parrots (Psittaciformes)". Sci Rep. 3: 2066. Bibcode:2013NatSR...3E2066L. doi:10.1038/srep02066. PMC 3691562. PMID 23797622.
- Robeck, T.R.; Steinman, K.J.; Gearhart, S.; Reidarson, T.R.; McBain, J.F.; Monfort, S.L. (1 August 2004). "Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1". Biology of Reproduction. 71 (2): 650–660. doi:10.1095/biolreprod.104.027961. PMID 15115725.
- Hargrove, John (22 March 2016). "I trained killer whales at SeaWorld for 12 years. Here's why I quit". Vox. Retrieved 25 February 2019.
- Hammond, John, et al., The Artificial Insemination of Cattle (Cambridge, Heffer, 1947, 61pp)
- Detailed description of the different fertility treatment options available
- A history of artificial insemination
- What are the Ethical Considerations for Sperm Donation?
- United States state court rules sperm donor is not liable for children
- UK Sperm Donors Lose Anonymity
- AI technique in the equine
- IntraUterine TuboPeritoneal Insemination (IUTPI)
- The Hastings Center's Bioethics Briefing Book entry on assisted reproduction
- Annales de Gembloux L´Organisation Scientifique de l Índustrie Animale en URSS, Artificial Insemination in the URSS, by Luis Thomasset, 1936
- More Information on Intrauterine Insemination