Imidacloprid

Imidacloprid is a systemic insecticide belonging to a class of chemicals called the neonicotinoids which act on the central nervous system of insects. The chemical works by interfering with the transmission of stimuli in the insect nervous system. Specifically, it causes a blockage of the nicotinergic neuronal pathway. By blocking nicotinic acetylcholine receptors, imidacloprid prevents acetylcholine from transmitting impulses between nerves, resulting in the insect's paralysis and eventual death. It is effective on contact and via stomach action.[1] Because imidacloprid binds much more strongly to insect neuron receptors than to mammal neuron receptors, this insecticide is more toxic to insects than to mammals.[2]

Imidacloprid[1]
Imidacloprid.svg
Imidacloprid.png
Names
IUPAC name
N-{1-[(6-Chloro-3-pyridyl)methyl]-4,5-dihydroimidazol-2-yl}nitramide
Identifiers
3D model (JSmol)
ChEBI
ChEMBL
ChemSpider
DrugBank
ECHA InfoCard 100.102.643 Edit this at Wikidata
KEGG
UNII
  • InChI=1S/C9H10ClN5O2/c10-8-2-1-7(5-12-8)6-14-4-3-11-9(14)13-15(16)17/h1-2,5H,3-4,6H2,(H,11,13) checkY
    Key: YWTYJOPNNQFBPC-UHFFFAOYSA-N checkY
  • InChI=1/C9H10ClN5O2/c10-8-2-1-7(5-12-8)6-14-4-3-11-9(14)13-15(16)17/h1-2,5H,3-4,6H2,(H,11,13)
    Key: YWTYJOPNNQFBPC-UHFFFAOYAZ
  • [O-][N+](=O)NC/1=N/CCN\1Cc2cnc(Cl)cc2
Properties
C9H10ClN5O2
Molar mass 255.661
Appearance Colorless crystals
Melting point 136.4 to 143.8 °C (277.5 to 290.8 °F; 409.5 to 416.9 K)
0.51 g/L (20 °C)
Pharmacology
QP53AX17 (WHO)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
checkY verify (what is checkY☒N ?)
Infobox references

As of 1999, imidacloprid was the most widely used insecticide in the world.[3] Although it is now off patent, the primary manufacturer of this chemical is Bayer CropScience (part of Bayer AG). It is sold under many names for many uses; it can be applied by soil injection, tree injection, application to the skin of the plant, broadcast foliar, ground application as a granular or liquid formulation, or as a pesticide-coated seed treatment.[4][2][5] Imidacloprid is widely used for pest control in agriculture. Other uses include application to foundations to prevent termite damage, pest control for gardens and turf, treatment of domestic pets to control fleas,[2] protection of trees from boring insects,[6] and in preservative treatment of some types of lumber products.[7]

Authorized usesEdit

Imidacloprid is the most widely used insecticide in the world.[citation needed] Its major uses include:

When used on plants, imidacloprid, which is systemic, is slowly taken up by plant roots and slowly translocated up the plant via xylem tissue.

Application to treesEdit

When used on trees, it can take 30–60 days to reach the top (depending on the size and height) and enter the leaves in high enough quantities to be effective. Imidacloprid can be found in the trunk, the branches, the twigs, the leaves, the leaflets, and the seeds. Many trees are wind pollinated. But others such as fruit trees, linden, catalpa, and black locust trees are bee and wind pollinated and imidacloprid would likely be found in the flowers in small quantities. Higher doses must be used to control boring insects than other types.[6]

BackgroundEdit

On January 21, 1986, a patent was filed and granted on May 3, 1988, for imidacloprid in the United States (U.S. Pat. No. 4,742,060) by Nihon Tokushu Noyaku Seizo K.K. of Tokyo, Japan.[10]

On March 25, 1992, Miles, Inc. (later Bayer CropScience) applied for registration of imidacloprid for turfgrass and ornamentals in the United States. On March 10, 1994, the U.S. Environmental Protection Agency approved the registration of imidacloprid.[11]

On January 26, 2005, the Federal Register notes the establishment of the '(Pesticide Tolerances for) Emergency Exemptions' for imidacloprid. It use was granted to Hawaii (for the) use (of) this pesticide on bananas(,) and the States of Minnesota, Nebraska, and North Dakota to use (of) this pesticide on sunflower(s).[12]

BiochemistryEdit

Imidacloprid is a systemic chloronicotinyl pesticide, belonging to the class of neonicotinoid insecticides. It works by interfering with the transmission of nerve impulses in insects by binding irreversibly to specific insect nicotinic acetylcholine receptors.[13]

As a systemic pesticide, imidacloprid translocates or moves easily in the xylem of plants from the soil into the leaves, fruit, pollen, and nectar of a plant. Imidacloprid also exhibits excellent translaminar movement in plants and can penetrate the leaf cuticle and move readily into leaf tissue.[14]

Since imidacloprid is efficacious at very low levels (nanogram and picogram), it can be applied at lower concentrations (e.g., 0.05–0.125 lb/acre or 55–140 g/ha) than other insecticides. The availability of imidacloprid and its favorable toxicity package as compared to other insecticides on the market in the 1990s allowed the EPA to replace more toxic insecticides including the acetylcholinesterase inhibitors, the organophosphorus compounds, and methylcarbamates.[15][16]

Environmental fateEdit

The main routes of dissipation of imidacloprid in the environment are aqueous photolysis (half-life = 1–4 hours) and plant uptake. The major photometabolites include imidacloprid desnitro, imidacloprid olefine, imidacloprid urea, and five minor metabolites. The end product of photodegradation is chloronicotinic acid (CNA) and ultimately carbon dioxide. Since imidacloprid has a low vapor pressure, it normally does not volatilize readily.[13]

Although imidacloprid breaks down rapidly in water in the presence of light, it remains persistent in water in the absence of light. It has a water solubility of .61 g/L, which is relatively high.[17] In the dark, at pH between 5 and 7, it breaks down very slowly, and at pH 9, the half-life is about 1 year. In soil under aerobic conditions, imidacloprid is persistent with a half-life of the order of 1–3 years. On the soil surface the half-life is 39 days.[18] Major soil metabolites include imidacloprid nitrosimine, imidacloprid desnitro and imidacloprid urea, which ultimately degrade to 6-chloronicotinic acid, CO2, and bound residues.[8][13] 6-Chloronicotinic acid is recently shown to be mineralized via a nicotinic acid (vitamin B3) pathway in a soil bacterium.[19]

In soil, imidacloprid strongly binds to organic matter. When not exposed to light, imidacloprid breaks down slowly in water, and thus has the potential to persist in groundwater for extended periods. However, in a survey of groundwater in areas of the United States which had been treated with imidacloprid for the emerald ash borer, imidacloprid was usually not detected. When detected, it was present at very low levels, mostly at concentrations less than 1 part per billion (ppb) with a maximum of 7 ppb, which are below levels of concern for human health. The detections have generally occurred in areas with porous rocky or sandy soils with little organic matter, where the risk of leaching is high — and/or where the water table was close to the surface.[20]

Based on its high water solubility (0.5-0.6 g/L) and persistence, both the U.S. Environmental Protection Agency and the Pest Management Regulatory Agency in Canada consider imidacloprid to have a high potential to run off into surface water and to leach into ground water and thus warn not to apply it in areas where soils are permeable, particularly where the water table is shallow.[8][13]

According to standards set by the environmental ministry of Canada, if used correctly (at recommended rates, without irrigation, and when heavy rainfall is not predicted), imidacloprid does not characteristically leach into the deeper soil layers despite its high water solubility (Rouchaud et al. 1994; Tomlin 2000; Krohn and Hellpointner 2002).[13] In a series of field trials conducted by Rouchaud et al. (1994, 1996), in which imidacloprid was applied to sugar beet plots, it was consistently demonstrated that no detectable leaching of imidacloprid to the 10–20 cm soil layer occurred. Imidacloprid was applied to a corn field in Minnesota, and no imidacloprid residues were found in sample column segments below the 0-15.2 cm depth segment (Rice et al. 1991, as reviewed in Mulye 1995).[8][13]

However, a 2012 water monitoring study by the state of California, performed by collecting agricultural runoff during the growing seasons of 2010 and 2011, found imidacloprid in 89% of samples, with levels ranging from 0.1 to 3.2 µg/L. 19% of the samples exceeded the EPA threshold for chronic toxicity for aquatic invertebrates of 1.05 µg/L. The authors also point out that Canadian and European guidelines are much lower (0.23 µg/L and 0.067 µg/L, respectively) and were exceeded in 73% and 88% of the samples, respectively. The authors concluded that "imidacloprid commonly moves offsite and contaminates surface waters at concentrations that could harm aquatic invertebrates".[21]

Health effectsEdit

The effects of imidacloprid on human health depend on the dose, duration, and frequency of exposure. The effects may also depend on the health of a person and environmental factors. People who might orally ingest acute amounts would experience emesis, diaphoresis, drowsiness and disorientation. This would need to be intentional since a large amount would need to be ingested to experience a toxic reaction.[2]

ToxicologyEdit

Based on laboratory rat studies, imidacloprid is rated as "moderately toxic" on an acute oral basis to mammals and low toxicity on a dermal basis by the World Health Organization and the United States Environmental Protection Agency (class II or III, requiring a "Warning" or "Caution" label). It is rated as an "unlikely" carcinogen and as weakly mutagenic by the U.S. EPA (group E). It is not listed for reproductive or developmental toxicity, but is listed on EPA's Tier 1 Screening Order for chemicals to be tested under the Endocrine Disruptor Screening Program (EDSP).[11][22] Tolerances for imidacloprid residues in food range from 0.02 mg/kg in eggs to 3.0 mg/kg in hops.[1]

MammalsEdit

Imidacloprid and its nitrosoimine metabolite (WAK 3839) have been well studied in rats, mice and dogs.

In dogs the LD50 is 450 mg/kg of body weight (i.e., in any sample of medium-sized dogs weighing 13 kilograms (29 lb), half of them would be killed after consuming 5,850 mg of imidacloprid, or about 15th of an ounce). The acute inhalation LD50 in rats was not reached at the greatest attainable concentrations, 69 milligrams per cubic meter of air as an aerosol, and 5,323 mg a.i./m3 of air as a dust.

In mammals, the primary effects following acute high-dose oral exposure to imidacloprid are mortality, transient cholinergic effects (dizziness, apathy, locomotor effects, labored breathing) and transient growth retardation. Exposure to high doses may be associated with degenerative changes in the testes, thymus, bone marrow and pancreas. Cardiovascular and hematological effects have also been observed at higher doses.

The primary effects of longer term, lower-dose exposure to imidacloprid are on the liver, thyroid, and body weight (reduction). Low- to mid-dose oral exposures have been associated with reproductive toxicity, developmental retardation and neurobehavioral deficits in rats and rabbits. Imidacloprid is neither carcinogenic in laboratory animals nor mutagenic in standard laboratory assays.[23]

It is not irritating to eyes or skin in rabbits and guinea pigs.[1]

BeesEdit

Imidacloprid is acutely toxic to honeybees: its LD50 ranges from 5 to 70 nanograms per bee.[24] Honeybee colonies vary in their ability to metabolize toxins, which explains this wide range. Imidacloprid is more toxic to bees than the organophosphate dimethoate (oral LD50 152 ng/bee) or the pyrethroid cypermethrin (oral LD50 160 ng/bee).[24] The toxicity of imidacloprid to bees differs from most insecticides in that it is more toxic orally than by contact. The contact acute LD50 is 0.024 µg active ingredient per bee.[25]

In laboratory studies, sublethal levels of imidacloprid have been shown to impair navigation, foraging behavior, feeding behavior, and olfactory learning performance in honeybees (Apis mellifera).[24][26][27][28][29][30][31][32] In general, however, despite the fact that many laboratory studies have shown the potential for neonicotinoid toxicity, the majority of field studies have found only limited or no effects on honeybees.[33]

In bumblebees, exposure to 10 ppb imidacloprid reduces natural foraging behaviour, increases worker mortality and leads to reduced brood development.[34][35]

BirdsEdit

Imidacloprid is considered acutely toxic to birds, and to cause avian reproductive toxicity.[13]

In bobwhite quail (Colinus virginianus), imidacloprid was determined to be moderately toxic with an 14-day LD50 of 152 mg a.i./kg. It was slightly toxic in a 5-day dietary study with an acute oral LC50 of 1,420 mg a.i./kg diet, a NOAEC of < 69 mg a.i./kg diet, and a LOAEC = 69 mg a.i./kg diet. Exposed birds exhibited ataxia, wing drop, opisthotonos, immobility, hyperactivity, fluid-filled crops and intestines, and discolored livers. In a reproductive toxicity study with bobwhite quail, the NOAEC = 120 mg a.i./kg diet and the LOAEC = 240 mg a.i./kg diet. Eggshell thinning and decreased adult weight were observed at 240 mg a.i./kg diet.[11][13]

Imidacloprid is highly toxic to four bird species: Japanese quail, house sparrow, canary, and pigeon. The acute oral LD50 for Japanese quail (Coturnix coturnix) is 31 mg a.i./kg bw with a NOAEL = 3.1 mg a.i./kg. The acute oral LD50 for house sparrow (Passer domesticus) is 41 mg a.i./kg bw with a NOAEL = 3 mg a.i./kg and a NOAEL = 6 mg a.i./kg. The LD50s for pigeon (Columba livia) and canary (Serinus canaria) are 25–50 mg a.i./kg. Mallard ducks are more resistant to the effects of imidacloprid with a 5-day dietary LC50 of > 4,797 ppm. The NOAEC for body weight and feed consumption is 69 mg a.i./kg diet. Reproductive studies with mallard ducks showed eggshell thinning at 240 mg a.i./kg diet.[11][13]

According to the European Food Safety Authority, imidacloprid poses a potential high acute risk for both herbivorous and insectivorous birds.[16] Chronic risk has not been well established.[13]

A 2014 observational study conducted in the Netherlands correlated declines in some bird populations with environmental imidacloprid residues, although it stopped short of concluding that the association was casual.[36]

Aquatic lifeEdit

Imidacloprid is highly toxic on an acute basis to aquatic invertebrates, with EC50 values = 0.037 - 0.115 ppm. It is also highly toxic to aquatic invertebrates on a chronic basis (effects on growth and movement): NOAEC/LOAEC = 1.8/3.6 ppm in daphnids; NOAEC = 0.001 in Chironomus midge, and NOAEC/LOAEC = 0.00006/0.0013 ppm in mysid shrimp.

Its toxicity to fish is relatively low;[1] however, the EPA has requested review of secondary effects on fish with food chains that include sensitive aquatic invertebrates.[8]

Plant lifeEdit

Imidacloprid has been shown to turn off some genes that some rice varieties use to produce defensive chemicals. While imidacloprid is used for control of the brown planthopper and other rice pests, there is evidence that imidacloprid actually increases the susceptibility of the rice plant to planthopper infestation and attacks.[37] Imidacloprid has been shown to increase the rate of photosynthesis in upland cotton temperatures above 36 degrees Celsius.[38]

RegulationEdit

European UnionEdit

In the mid to late 1990s, French beekeepers reported a significant loss of bees, which they attributed to the use of imidacloprid.[citation needed] In 1999, the French Minister of Agriculture suspended the use of imidacloprid on sunflower seeds and appointed a team of expert scientists to examine the impact of imidacloprid on bees. In 2003, this panel issued a report which concluded that imidacloprid posed a significant risk to bees.[39] In 2004, the French Minister of Agriculture suspended the use of imidacloprid as a seed treatment for sunflowers and maize (corn). Certain imidacloprid seed treatments were also temporarily banned in Italy, following preliminary monitoring studies that identified correlations between bee losses and the use of neonicotinoid pesticides.[40]

In January 2013, a European Food Safety Authority (EFSA) report concluded that neonicotinoids posed an unacceptably high risk to bees: "A high acute risk to honey bees was identified from exposure via dust drift for the seed treatment uses in maize, oilseed rape and cereals. A high acute risk was also identified from exposure via residues in nectar and/or pollen."[16] The EFSA also identified a number of gaps in the scientific evidence and were unable to finalize risk assessments for some uses authorized in the European Union (EU). Following the report, EU member states voted to restrict the use of the three main neonics, including imidacloprid, for seed treatment, soil application (granules) and foliar treatment in crops attractive to bees.[41]

In February 2018, the European Food Safety Authority published a further report concluding that neonicotinoids posed a serious danger to bees.[16] In April 2018, the member states of the EU decided to ban the neonicotinoids for all outdoor uses.[42]

RegistrationsEdit

Among others, imidacloprid is sold under the brand names: Confidor (Bayer CropScience India),[43] Marathon (OHP, US).[44][45]

See alsoEdit

ReferencesEdit

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  2. ^ a b c d e f Gervais et al. 2010.
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  4. ^ "Imidacloprid: Human Health and Ecological Risk Assessment. Final Report" (PDF). USDA Forest Service. 2005. Retrieved July 23, 2021.
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  9. ^ Preston, Richard (2007). "A Death in the Forest". The New Yorker.
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  14. ^ Environmental Fate of Imidacloprid Archived March 16, 2012, at the Wayback Machine California Department of Pesticide Regulation 2006
  15. ^ "Imidacloprid: Risk Characterization Document - Dietary and Drinking Water Exposure" (PDF). California Environmental Protection Agency. February 9, 2006. Retrieved April 7, 2012.
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  17. ^ Flores-Céspedes, Francisco; Figueredo-Flores, Cristina Isabel; Daza-Fernández, Isabel; Vidal-Peña, Fernando; Villafranca-Sánchez, Matilde; Fernández-Pérez, Manuel (January 18, 2012). "Preparation and Characterization of Imidacloprid Lignin–Polyethylene Glycol Matrices Coated with Ethylcellulose". Journal of Agricultural and Food Chemistry. 60 (4): 1042–1051. doi:10.1021/jf2037483. PMID 22224401.
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  20. ^ Hahn, Jeffrey; Herms, Daniel A.; McCullough, Deborah G. (February 2011). "Frequently Asked Questions Regarding Potential Side Effects of Systemic Insecticides Used To Control Emerald Ash Borer". University of Michigan Extension, Michigan State University, The Ohio State University Extension.
  21. ^ Starner, Keith; Goh, Kean S. (2012). "Detections of Imidacloprid in Surface Waters of Three Agricultural Regions of California, USA, 2010-2011". Bulletin of Environmental Contamination and Toxicology. 88 (3): 316–321. doi:10.1007/s00128-011-0515-5. PMID 22228315. S2CID 18454777.
  22. ^ Endocrine Disruptor Screening Program: Tier 1 Screening Order Issuing Announcement. Federal Register Notice, Oct 21, 2009. Vol. 74, No. 202, pp. 54422-54428
  23. ^ USDA, Forest Service, Forest Health Protection (December 28, 2005). Imidacloprid - Human Health and Ecological Risk Assessment – Final Report "HUMAN HEALTH RISK ASSESSMENT / Overview. 3-1". United States Forest Service. Retrieved July 30, 2013.[dead link]
  24. ^ a b c Suchail, Séverine; Guez, David; Belzunces, Luc P. (November 2001). "Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera". Environmental Toxicology and Chemistry. 20 (11): 2482–2486. doi:10.1002/etc.5620201113. PMID 11699773. S2CID 22209995.
  25. ^ Suchail, Séverine; Guez, David; Belzunces, Luc P. (July 2000). "Characteristics of imidacloprid toxicity in two Apis mellifera subspecies" (PDF). Environmental Toxicology and Chemistry. 19 (7): 1901–1905. doi:10.1002/etc.5620190726.
  26. ^ Armengaud, C.; Lambin, M.; Gauthier, M. (2002), "Effects of imidacloprid on the neural processes of memory", in Devillers, J; Pham-Delegue, M.H. (eds.), Honey bees: estimating the environmental impact of chemicals, New York: Taylor & Francis, pp. 85–100, ISBN 9780415275187
  27. ^ Decourtye, A; Lacassie, E; Pham-Delegue, M-H (2003). "Learning performances of honeybees (Apis mellifera L) are differentially affected by imidacloprid according to the season". Pest Manag. Sci. 59 (3): 269–278. doi:10.1002/ps.631. PMID 12639043.
  28. ^ Decourtye, A.; Armengaud, C.; Devillers, R.M.; Cluzeau, S. (2004). "Imidacloprid impairs memory and brain metabolism in the honeybee (Apis mellifera L)". Pesticide Biochem Phys. 78 (2): 83–92. doi:10.1016/j.pestbp.2003.10.001.
  29. ^ Guez, D.; Suchail, S.; Gauthier, M.; Maleszka, R.; Belzunces, L. (2001). "Contrasting effects of imidacloprid on habituation in 7- and 8-day old honeybees (Apis mellifera)". Neurobiology of Learning and Memory. 76 (2): 183–191. doi:10.1006/nlme.2000.3995.
  30. ^ Pham-Delegue, M.H.; Cluzeau, S. (1999), "Effets des produits phytosanitaires sur l'abeille; incidence du traitement des semences de tournesol par Gaucho sur les disparitions de butineuses", Rapport final de synthese au Ministere de l'Agriculture et de la Peche
  31. ^ Lambin, M.; Armengaud, C.; Ramond, S.; Gauthier, M. (2001). "Imidacloprid-induced facilitation of the proboscis extension reflex habituation in the honeybee". Archives of Insect Biochemistry and Physiology. 48 (3): 129–134. doi:10.1002/arch.1065.
  32. ^ Williamson, S.M.; Wright, G.A (2013). "Exposure to multiple cholinergic pesticides impairs olfactory learning and memory in honeybees" (PDF). Journal of Experimental Biology. 216 (10): 1799–1807. doi:10.1242/jeb.083931. ISSN 0022-0949. PMC 3641805. PMID 23393272.
  33. ^ "Neonicotinoids". Pollinator Network @ Cornell. Retrieved May 10, 2019.
  34. ^ Gill R.J.; Ramos-Rodriguez O.; Raine N.E. (2012). "Combined pesticide exposure severely affects individual-and colony-level traits in bees". Nature. 491 (7422): 105–108. doi:10.1038/nature11585. PMC 3495159. PMID 23086150.
  35. ^ Bryden, John; Gill, Richard J.; Mitton, Robert A. A.; Raine, Nigel E.; Jansen, Vincent A. A. (2013). "Chronic sublethal stress causes bee colony failure". Ecology Letters. 16 (12): 1463–1469. doi:10.1111/ele.12188. PMC 4299506. PMID 24112478.
  36. ^ Hallmann CA, Foppen RP, van Turnhout CA, de Kroon H, Jongejans E (July 2014). "Declines in insectivorous birds are associated with high neonicotinoid concentrations". Nature. 511 (7509): 341–3. Bibcode:2014Natur.511..341H. doi:10.1038/nature13531. PMID 25030173. S2CID 4464169.
  37. ^ Cheng Y, Shi ZP, Jiang LB, Ge LQ, Wu JC, Jahn GC (March 2012). "Possible connection between imidacloprid-induced changes in rice gene transcription profiles and susceptibility to the brown plant hopper Nilaparvatalugens Stål (Hemiptera: Delphacidae)". Pestic Biochem Physiol. 102–531 (3): 213–219. doi:10.1016/j.pestbp.2012.01.003. PMC 3334832. PMID 22544984.
  38. ^ Gonias, Evangelos D.; Oosterhuis, Derrick M.; Bibi, Androniki C. (2007). "Physiologic Response of Cotton to the Insecticide Imidacloprid under High-Temperature Stress". Journal of Plant Growth Regulation. 27 (1): 77–82. doi:10.1007/s00344-007-9033-4. ISSN 0721-7595. S2CID 20930112.
  39. ^ Comité Scientifique et Technique (18 September 2003). "Imidaclopride utilisé en enrobage de semences (Gaucho) et troubles des abeilles: Rapport final" [Imidacloprid used in coating seeds (Gaucho) and disorders of bees: Final report] (PDF) (in French). Archived from the original (PDF) on 16 March 2012. Retrieved 18 April 2012.
  40. ^ "Colony Collapse Disorder: European Bans on Neonicotinoid Pesticides - Pesticides - US EPA". epa.gov. June 23, 2010. Archived from the original on September 4, 2011. Retrieved July 24, 2021.
  41. ^ McDonald-Gibson, Charlotte (29 April 2013). "'Victory for bees' as European Union bans neonicotinoid pesticides blamed for destroying bee population". The Independent. Archived from the original on 1 May 2013. Retrieved 1 May 2013.
  42. ^ "EU to fully ban neonicotinoid insecticides to protect bees". Reuters. 27 April 2018. Retrieved 29 April 2018.
  43. ^ "Insecticide-Confidor". Bayer CropScience India. Retrieved 2021-04-11.
  44. ^ US EPA (United States Environmental Protection Agency) (2015-04-21). "Label Amendment - minor label revisions Product Name: Marathon 1% Granular Greenhouse and Nursery Insecticide EPA Registration Number: 59807-15 Application Date: March 17, 2015 Decision Number: 502678" (PDF). Archived from the original (PDF) on 2021-04-11.
  45. ^ OHP. "MARATHON 1% Granular" (PDF). Archived from the original (PDF) on 2018-05-16.

External linksEdit