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DescriptionHis-tag-primers.png
English: Example of primer designed to add a 6xHis-tag using PCR. Eighteen bases coding six histidines are inserted right after the STAR codon or right before the STOP codon. At least 16 bases specific of a gene of interest are need it next to the His-tag.
AAAsBiophysics 20:27, 17 July 2007 (UTC)
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2007-07-17 19:38 Jfhaller 565×106× (7770 bytes) Example of primer designed to add a 6xHis-tag using PCR. Eighteen bases coding six histidines are inserted right after the STAR codon or right before the STOP codon. At least 16 bases specific of a gene of interest are need it next to the His-tag.
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