EM Data Bank

  (Redirected from EMDB)

The EM Data Bank or Electron Microscopy Data Bank (EMDB) collects 3D EM maps and associated experimental data determined using electron microscopy of biological specimens. It was established in 2002 at the MSD/PDBe group of the European Bioinformatics Institute (EBI), where the European site of the EMDataBank.org consortium is located.[2] As of 2015, the resource contained over 2,600 entries with a mean resolution of 15Å.[3]

EMDataBank.org
Database.png
Content
DescriptionUnified Data Resource for CryoEM.
Contact
Research centerEuropean Bioinformatics Institute (UK site) AND Rutgers University (USA site)
LaboratoryPDBe & RCSB PDB
Primary citationLawson & al. (2011)[1]
Release date2002
Access
Websiteebi.ac.uk/emdb

Deposition of data was originally via the EMdep deposition interface, but since 2016 deposition of data has been incorporated into the wwPDB OneDep interface[4]

Under the NIH Unified Data Resource for CryoEM, the Research Collaboration for Structural Biology (RCSB) also acts as a deposition, data processing and distribution center for EMDB data, while the National Center for Macromolecular Imaging (NCMI) is a collaborative partner in providing services and tools concerning the EMDB.

EM Data Bank also provides the EMsearch search tool and data can also be queried at RCSB, EMBL-EBI and PDBj.

EMDB is an archive for three-dimensional density maps of all types of biological assemblies, including ribosomes, chaperones, polymerases, multifunctional enzymes and viruses. Viper EMDB at Scripps is a separate database for three-dimensional EM maps of viruses.

To compare and assess methods for the new generation of higher resolution (better than 5Å) structures, the EMDB has hosted the first CryoEM Map Challenge and CryoEM Model Challenge, reported in a special issue of the Journal of Structural Biology.[5]

See alsoEdit

ReferencesEdit

  1. ^ Lawson, Catherine L; Baker, Matthew L; Best, Christoph; et al. (January 2011). "EMDataBank.org: unified data resource for CryoEM". Nucleic Acids Research. 39 (suppl 1): D456-464. doi:10.1093/nar/gkq880. PMC 3013769. PMID 20935055.
  2. ^ EMDB. "Electron Microscopy Data Bank". Electron Microscopy Data Bank. Retrieved 2021-08-05.
  3. ^ Esquivel-Rodríguez, J; Xiong, Y; Han, X; et al. (30 May 2015). "Navigating 3D electron microscopy maps with EM-SURFER". BMC Bioinform. 16 (181). doi:10.1186/s12859-015-0580-6. PMC 4448178. PMID 26025554.
  4. ^ Young, JY; Westbrook, JD; Feng, Z; Sala, R; Peisach, E; Oldfield, TJ; Sen, S; Gutmanas, A; Armstrong, DR; Berrisford, JM; Chen, L; Chen, M; Di Costanzo, L; Dimitropoulos, D; Gao, G; Ghosh, S; Gore, S; Guranovic, V; Hendrickx, PM; Hudson, BP; Igarashi, R; Ikegawa, Y; Kobayashi, N; Lawson, CL; Liang, Y; Mading, S; Mak, L; Mir, MS; Mukhopadhyay, A; Patwardhan, A; Persikova, I; Rinaldi, L; Sanz-Garcia, E; Sekharan, MR; Shao, C; Swaminathan, GJ; Tan, L; Ulrich, EL; van Ginkel, G; Yamashita, R; Yang, H; Zhuravleva, MA; Quesada, M; Kleywegt, GJ; Berman, HM; Markley, JL; Nakamura, H; Velankar, S; Burley, SK. "OneDep: Unified wwPDB System for Deposition, Biocuration, and Validation of Macromolecular Structures in the PDB Archive". Structure. 25: 536–545. doi:10.1016/j.str.2017.01.004. PMC 5360273. PMID 28190782.
  5. ^ Lawson, Catherine; Chiu, Wah, eds. (2018). "The CryoEM Structure Map and Model Challenges". Journal of Structural Biology. 204 (1). ISSN 1047-8477.

External linksEdit