His brothers Benedetto Ghirlandaio (1458–1497) and Domenico Ghirlandaio (1449–1494) were both painters, as was his nephew Ridolfo Ghirlandaio (1483–1561). Davide was an assistant to, and then a partner of, his brother, the leading painter Domenico. After Domenico's death, Davide took over the studio and trained Domenico's son Ridolfo. He was active in the mosaic decoration of the Orvieto Cathedral.
- Dizionario enciclopedico Bolaffi dei pittori e degli incisori italiani dall'XI al XX secolo, Turin, Giulio Bolaffi, 1972–1976.
- Vasari, Giorgio, Le Vite delle più eccellenti pittori, scultori, ed architettori, many editions and translations.
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