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Columella is Latin meaning "little column". It may refer to:
- Columella (4 - ca. 70), a Roman writer
- Columella (gastropod), an anatomical feature of a coiled snail shell or gastropod shell.
- Columella (genus), a genus of land gastropods, in the family Vertiginidae.
- Columella (botany), in mycology and bryology, the central sterile portion of the sporangium, found in various fungi as well as in mosses and hornworts.
- In corals, the central axis structure of a corallite formed by the inner ends of the septa.
- In human anatomy, Columella nasi, the fleshy external end of the nasal septum.
- Columella (auditory system), a part of the auditory system of amphibians, reptiles and birds, corresponding to the stapes in mammals.
- Columella (root), in plant anatomy, a distinct tissue layer in the very tip of the root. It is a column of cells below the quiescent centre (QC = stem cells in the growing tip of the root).