Cell physiology (including cellular electrophysiology) is the biological study (which has been a very indispensable study in the last several years) about the activities that take place in a cell to keep it alive. This includes, among animal cells, plant cells and microorganisms. The term "physiology" refers to all the normal functions that take place in a living organism. All of these activities in the cell could be counted as following ; nutrition, environmental response , cell growth , cell division , reproduction and differentiation. The differences among the animal cell, plant cell and microorganisms shows the essential functional similarity eventhough those cells have different structures. Absorption of water by roots, production of food in the leaves, and growth of shoots towards light are examples of plant physiology. The heterotrophic metabolism of food derived from plants and animals and the use of movement to obtain nutrients (even if the organism itself remains in a relatively stationary position) are characteristic of animal physiology.
In the context of human physiology, the term cell physiology often specifically applies to the physiology of membrane transport, neuron transmission, and (less frequently) muscle contraction. In general, these cover the digestion of food, circulation of blood, and contraction of muscles and, therefore, are important aspects of human physiology. For a more complete description of the general physiological function of human cells (as well as the cells of other life forms), see the article on cell biology.
Experimental approach in cell physiology is an important aspect in cell physiology because it utilizes the experimental methods in order to solve any scientific issue related to physiology. the following examples have been studied in cellular problems by using the experimental method: First, the key of understanding cells' activities in animal, plant cells and microorganisms was studied by identified the nature of organization of cells. Second, the differences of the environment plays a role of the nature of the cell environment, cell resistance and the adjustment. Third, the nature of the cell in regulating and transporting materials into and out the cells crossing the cell membrane. Fourth, Cell foods and its inter-conversions and the mechanism of respiration process to release energy from cell's food. Fifth, the use of energy in respiration in terms of performing the variety types of work. For example, maintenance, readiness, osmotic and for manufacturing of secretions.
The relation of cell physiology to different fields of physiology such as, animal physiology, comparative animal physiology, plant physiology and molecular biology are being fundamental parts of physiology field. Animal physiology plays a role of the work of various organs of the body which those organs coordinate to integrate the animal behavior. medical men in were concerned about vertebrate organ physiology precisely with mammals, because the information provided could be beneficially applied to the physiology of human in health and diseases. it is a beneficial to work on organ physiology as an medical approach. However, the Cellular level can be used as well. In addition, Comparative animal physiology is a prat of studying the function of any organ in various types of animals such as, vertebrate and invertebrate to find the fundamental relations. Plant physiology is more likely concerns about the response, nutrition, growth and reproduction of different types of plants. and Since the functioning of the animal and plant depend on the function of the cells component. All of these physiological studies have been recognized by researchers whom were interested in organisms and worked at the cellular level to solve problems at the organs levels. on the other hand, Molecular biology turns to explain the cells activities in at the molecular level.In the past time, it has been limited in studying activities of viruses and bacteria. However, now it is being extended on studying the activities of the Eukaryota cells. Molecular biology has been contributed as an aim for cell physiology by being as powerful source of mutants (this method has been applied to many issues in cell physiology) such as, transportation across the cell membrane and the nature of the membranes. However, many of the cell physiology problems have not been solved by the molecular approach.
General characteristics of cell physiologyEdit
Prokaryotes first came into existence and contain no self-contained nucleus, therefore making their mechanisms much simpler compared to the later-evolved Eukaryotes, which do contain a nucleus enveloping the cell's DNA and nuclear organelles. Because viruses, viroids, prions and such (see Acytota/Aphanobionta) depend entirely on the physiology of other cells (i.e., cells containing their own physiology), the former entities are often not considered to be "living" by the biologists who study them.
All living cells, whether prokaryotes or eukaryotes, contain the following distinguishing characteristics:
- The genetic code is based on DNA.
- The DNA is composed of four nucleotides (deoxyadenosine, deoxycytidine, deoxythymidine and deoxyguanosine), to the exclusion of other possible deoxynucleotides.
- The genetic code is composed of three-nucleotide codons, thus producing 64 different codons. Since only 20 amino acids are used, multiple codons code for the same amino acids. This structure is arbitrary and shared by all eukaryotes and prokaryotes. Archaea and mitochondria use a similar code with minor differences.
- The DNA is kept double-stranded by a template-dependent DNA polymerase.
- The integrity of the DNA is maintained by a group of maintenance enzymes, including DNA topoisomerase, DNA ligase and other DNA repair enzymes. The DNA is also protected by DNA-binding proteins like histones.
- The genetic code is expressed via RNA intermediates, which are single-stranded.
- RNA is produced by a DNA-dependent RNA polymerase using nucleotides similar to those of DNA with the exception of Thymidine in DNA, replaced by Uridine in RNA.
- The genetic code is expressed into proteins. All other properties of the organism (e.g. synthesis of lipids or carbohydrates) are the result of protein enzymes.
- Proteins are assembled from free amino acids by translation of an mRNA by ribosomes, tRNA and a group of related proteins.
- Only 20 amino acids are used, to the exclusion of countless non-standard amino acids; only the L-isomers are used.
- Amino acids must be synthesized from glucose by a group of specialized enzymes; the synthesis pathways are arbitrary and conserved.
- Glucose can be used as a source of energy and carbon; only the D-isomer is used.
- Glycolysis goes through an arbitrary degradation pathway.
- ATP is used as an energy intermediate.
- The cell is surrounded by a cellular membrane composed of a lipid bilayer.
- Inside the cell, the concentration of sodium is lower, and potassium is higher, than outside. This gradient is maintained by specific ion pumps.The concentration of Calcium inside of a cell is also lower than outside.
- The cell multiplies by duplicating all its contents followed by cellular division.
The earliest ancestor of all life that is hypothesized to contain these attributes is known as the last common ancestor.
- Movement of proteins — The movement of proteins about the cell for use in structure and in enzymatic processes.
- Active transport and Passive transport — The processes facilitating the movement of molecules into and out of cells.
- Autophagy — The process whereby cells "eat" their own internal components or microbial invaders.
- Adhesion — The chemical processes whereby cells and other tissues are held together.
- Cell division — a eukaryotic cell process resulting in the formation of daughter cells; there are two major types: that of mitosis (asexual reproduction) and meiosis (sexual reproduction).
- Cell movement: Chemotaxis, Contraction, Cilia and Flagella.
- Cell signaling — Regulation of cell behavior by signals from outside, such as the use of hormones or neurotransmitters.
- DNA repair and Cell death
- Metabolism: Glycolysis, Respiration, Photosynthesis — Processes whereby energy is stored and/or liberated for use by the cell.
- Transcription and mRNA splicing — Processes by which genes express themselves, primarily by way of RNA and protein transcription.
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