Botsfengselet is a former national prison for long-term prisoners in Oslo, Norway. Its location is at the former Åkebergløkka, Grønlandsleiret 41.
The prison was designed by architect Heinrich Ernst Schirmer. The prison was built starting 1844 and came into use from 1851. The prison chapel was designed by architect Jacob Wilhelm Nordan and came into use during 1887.
In 1970 Ullersmo Prison took over as a prison for long-term prisoners in Norway, while Botsfengselet was rehabilitated and served as a department of the district prison in Oslo (Norwegian: Oslo Kretsfengsel) from 1975. The prison is listed as a protected site.