A bacteriologist is a microbiologist or a professional[1] trained in bacteriology, a subdivision of microbiology. The duties of a bacteriologist include prevention, diagnosis and prognosis of diseases, as well as health care, and they may carry out various functions such as epidemiological surveillance, quality auditing with biotechnology development, basic research, management and teaching related to the career, scientist management, laboratory coordination and blood banks.[2]

Staphylococcus aureus (AB Test)

Official nameEdit

As the first acknowledged microscopist and microbiologist in history, Antonie van Leeuwenhoek was the first to undisputedly discover (observe), study, describe, conduct scientific experiments with a large array of microscopic organisms (including bacteria, which he called “animalcules”) and relatively determine their size, using single-lensed microscopes of his own design.[3][4][5][6][7][8]

The name can vary depending on the official laws of each country and how they are established in the society and therefore they would have a different official name. Whether they have different official name among the countries, the background almost is similar.

Country Diploma Official name
Canada Laboratory Technician & histopathologist Laboratory Technician & histopathologist[9]
China Bacteriologist and/or Clinical Laboratory Technician Bacteriologist
Other countries i.e. Chile, Mexico, Spain Biomedicine Biomedical Scientist
Russia Graduates in bioanalysis Bioanalyst[10]

Called Medical Laboratory Scientist or Bachelor of Science in the United States depending on their training, bacteriologists to homologate their title in the United States are called Bachelor of Bacteriology.


The main aim of a bacteriologist is prognosticate, diagnose and the disease surveillance by a huge range of laboratory test, all of them in the context of health care. Hence, a bacteriologist plays a role in research, management of the health care, promoting health and disease prevention.

There are plenty of methods and procedures used by the bacteriologist in the clinical laboratory whose purpose is the diagnosis of various diseases, some of these techniques are:

Techniques in Clinical Chemistry: used for determination of various analyses in various biological fluids, different reagents are used to play colorimetric techniques and enzyme kinetics, among others. The result of these reactions were measured by spectrometer or other similar physical techniques to quantitatively determine the value of an analyte, so as measured quantitatively analytes such as creation, BUN, lipid profile, total protein, glucose, direct bilirubin and overall, among others.

Techniques in Immunology: many of the immunological techniques used in clinical laboratory are based on the ability of antibodies to bind to specific antigens either in vitro or vivo, is how this feature that is exploited by the professional laboratory diagnose various diseases and / or semi-quantitative or quantitative determination of various analytes, among the various techniques used in immunology section include: immunology Test as ELISA (Enzyme-linked immunodeficient assay) for its acronym in English, with agglutination techniques latex particles coated with antibody or antigen, agglutination techniques, articulation chromatography techniques, microorganisms stereotyping techniques, fluorescence, among many others. Some of the tests performed in this section mentioned techniques are: rheumatoid factor, antinuclear antibodies by agglutination with latex particles, c-reactive protein, chromatography for determination of Human Chorionic Gonadotropin, HIV 1 and 2, hepatitis B virus, ELISA for determination of hormones and the presence of other microorganisms causing diseases among other tests.

Techniques in Microbiology: mainly used to determine infectious diseases the causative agent of the disease, regardless of whether it is caused by a bacterium, virus, fungi or parasites, bacteriologist through various coloration's, microscopic observation, specific crops for growth of each organism, biochemical tests whether manual, semi-automated or automated used to determine the genus and species of the organism causing the disease. a bacteriologist should be able to identify and name any parasitic observe microscopic structure, recently antimicrobial susceptibility tests have become very important for the emergence of multi-resistant bacterial strains in clinical microbiology section of the laboratory tests performed bacteriologists antimicrobial susceptibility to determine which antibiotics should treat various infectious diseases.

Techniques in Hematology: Section of Hematology various examinations are performed as: Table He mic, FSP (peripheral blood smear), reticulated count, observation and identification of parasites, determining blood type and RH, erythrocyte sedimentation rate, among other . Bacteriologists in specialized clinical laboratory hematology specialists perform more specific tests for diagnosis of hematologic diseases such as leukemia, anemia, regardless of etiology or other genetic alterations by various techniques such wherein phenotype cell markers studies performed by flow optometry, molecular biology techniques molecular as electroscopes, PCR and other tests such as: osmotic fragility, analysis of bone marrow aspirates, among others.

Techniques in Molecular Biology and Genetics: in specialized clinical laboratory services are included with molecular biology and genetics where the bacteriologist makes techniques such as chain reaction (PCR), electroscopes, Southern blot, Western blot, and DNA sequencing techniques genetically among others, which can be quite useful for diagnosis of various diseases.

Techniques in Blood Bank and Transfusion Unit: Section blood bank and / or transfusion unit performs professional laboratory testing donated blood units to ensure they are in good condition to be transfused to a patient, tests are done as presence or absence of infectious agents that produce diseases such as HIV, Sagas disease, Hepatitis, HTLV I, syphilis and others. Equally prepared and necessary processes are separated by centrifugal units of RBCs, precipitate, and platelet units reductionist procedures among others. Different unit processes in transfusion procedures are performed to measure the compatibility of the donor - recipient, the bacteriologist must verify the absence of antibodies that produce severe thrombolytic reactions at the time of transfusion between these procedures include: cross match minor, major crosspatch, determining blood type and RH, direct Combs, Combos indirect detection of antibodies and antibodies.

All manual or automated procedures require the strictest quality control. Checks should be made internal and external quality, make the appropriate calibrations, maintenance of equipment when necessary and properly validate every bacteriologist should follow the protocols are conducted in the clinical laboratory for quality control concerns, besides being constantly updated theoretical and academic in this way make a correct clinical correlation and ensure the accuracy of the results reported to the doctor.


One of the most important properties that should be a bacteriologist is his biosecurity. A bacteriologist should work with the right equipment depending on the level of the laboratory where the work and the danger of working with samples, this equipment includes: gown, masks, goggles biosecurity, latex gloves, biohazard cabinet, if it is a laboratory where highly dangerous microorganisms work bacteriologist must use special biosafety suits.

Some of the samples analyzed by bacteriologists are:

  • Blood samples
  • Samples of suppurative lesions
  • Samples of cerebrospinal fluid
  • Pleural fluid samples
  • Samples of ascitic fluid
  • Tissue infected
  • Samples of synovial fluid
  • Samples for urinalysis
  • Samples for stool tests
  • Samples of parasitic structures


Many medical diagnoses are based on the various examinations conducted by the bacteriologist in the clinical laboratory

A bacteriologist has general knowledge in bacteriology, hematology, parasitology, mycology, virology, immunology, genetics, cytogenetics, molecular biology, quality control, biotechnology, among other disciplines. The bacteriologist can reinforce the knowledge acquired through specialization, diploma, masters and doctoral degrees recognized by official institutions.

A bacteriologist performs in different fields as either professional or coordinator of clinical laboratory and blood banks, scientific research groups, microbiological quality control, quality control in clinical laboratory equipment epidemiology and public health, clinical laboratory veterinarian forensic clinical laboratory teaching in the fields of competence, among others. Besides this is the duty of bacteriologist transmit their knowledge to practitioners and bacteriologists future clinical laboratory professionals.


  • Passion for the profession.
  • Social Status
  • Conduct research across the breadth of his knowledge.
  • Perform various specializations (Hematology, Microbiology, Clinical Chemistry, Genetics, Cytogenetics, Immunology, etc. degree in various fields of life sciences and medical commitment to the medical staff.
  • Providing accuracy, precision, reliability, etc. of the obtained results.
  • Opportunities to expand their knowledge to any branch of biology.
  • Compensation of the work.
  • Size of workplace (clinical laboratory in different specialties, research, quality control, clinical epidemiology, public health, industry, forensics lab, among others.


A bacteriologist have the obligation of have the following attitudes;

  • Good reception and attention of the patients.
  • Complete monitoring of the samples.
  • To save the professional secrecy.
  • To know and implement the profession ethic code established by the authorities.

Professional careerEdit

In Colombia is regulated by the Bacteriologist National School Colegio Nacional de Bacteriólogo (CNB)”[11] which determine whether the professional is ready or not to perform the professional career by the obtaining of a professional card. that qualify to the professional to perform the professional career, it is regulated by the law (Article 2 of Act 1193 of 2008 which amends Act 841 of 2003), which determines; "The National Association of Bacteriologists assumes the functions of issuing of the professional card referred in Article 5 of Act, the Professional Cards, meanwhile inscriptions or records Bacteriologists will be issued by the Ministries of Health of the different departments"

“While the National Association of Bacteriologists acts as the issue of the professional card referred to in Article 5 of this Law, the Professional Cards, inscriptions or records Bacteriologists will be issued by the Ministries of Health of the different departments”[2]

In Bioethical see National Court and Ethics Bacteriology know will handle disciplinary proceedings on appeal - Bioethics - ethics - which identify professionals who practice in the profession of bacteriology in Colombia for petty offenses established in the existing laws matter. under Article 8 of Law 1193 of 2008.


Currently, to carry out the career in a different country of the initial studies, it is necessary to do a type approval or validation. As long as the professional be interested to work in the destiny country.

It is not necessary the approval or validate, when the professionals pretend to improve the knowledge in a Hague member country (Hague Convention), As long as the interested provided the correct documents legalization performed by the apostille convention, thus as ask for the postgraduates certificate access in accordance with paragraph c of Article 14 of Act 30 of 1992 (within Colombia),[12] It is necessary determine whether the destiny country is on the haya member list.[13]

Bacteriologist's dayEdit

In Colombia, 28 April is celebrated as Bacteriologist's day. However, in other countries this does not exist or is not celebrated. An international day is not present.

Noted bacteriologistsEdit

Laws and actsEdit

This are some of the laws/acts that regulated to bacteriologist; (Spanish)


Further information is on the web ite of “CNB”,[11] Colombian universities or you can check each of the Laws/Acts given above.[2][9][14][15][16]

See alsoEdit


  1. ^ "Bacteriologist: Job Description, Duties and Salary". Retrieved 2017-04-03.
  2. ^ a b c "Act 841 of 2003" (PDF). By which the exercise of the profession is regulated bacteriologist, // the code of bioethics and other provisions are issued (in Spanish). Ministry of Education. Retrieved 15 May 2014.
  3. ^ Dobell, Clifford (1932). Antony van Leeuwenhoek and His "Little Animals": being some account of the father of protozoology and bacteriology and his multifarious discoveries in these disciplines (Dover Publications ed.). New York: Harcourt, Brace and Company.
  4. ^ Corliss, John O (1975). "Three Centuries of Protozoology: A Brief Tribute to its Founding Father, A. van Leeuwenhoek of Delft". The Journal of Protozoology. 22 (1): 3–7. doi:10.1111/j.1550-7408.1975.tb00934.x. PMID 1090737.
  5. ^ Ford, Brian J. (1992). "From Dilettante to Diligent Experimenter: a Reappraisal of Leeuwenhoek as microscopist and investigator". Biology History. 5 (3).
  6. ^ Toledo-Pereyra, Luis H.: The Strange Little Animals of Antony van Leeuwenhoek — Surgical Revolution, in Surgical Revolutions: A Historical and Philosophical View. (World Scientific Publishing, 2008, ISBN 978-9814329620)
  7. ^ Lane, Nick (6 March 2015). "The Unseen World: Reflections on Leeuwenhoek (1677) 'Concerning Little Animal'". Philos Trans R Soc Lond B Biol Sci. 370 (1666): 20140344. doi:10.1098/rstb.2014.0344. PMC 4360124. PMID 25750239.
  8. ^ Chung, King-thom; Liu, Jong-kang: Pioneers in Microbiology: The Human Side of Science. (World Scientific Publishing, 2017, ISBN 978-9813202948). "We may fairly call Leeuwenhoek “The first microbiologist” because he was the first individual to actually culture, see, and describe a large array of microbial life. He actually measured the multiplication of the bugs. What is more amazing is that he published his discoveries."
  9. ^ a b "Federal Education Act No. 24195/93 and its equivalent in the Province Act N ° 9330/01" (PDF). Establish a new structure of the education system Argentina (in Spanish). Governor. Retrieved 15 May 2014.
  10. ^ "Law of the exercise Bioanalysis within Venezuela" (PDF). Venezuela (in Spanish). Ministry of Health and Social Welfare Surveillance exercise of Bioanalysis. Archived from the original (PDF) on 14 May 2014. Retrieved 15 May 2014.
  11. ^ a b "Bacteriologist National School". Colombia (in Spanish). Retrieved 15 May 2014.
  12. ^ "Act 30 of 1992" (PDF) (in Spanish). National Accreditation Council, Republic of Colombia. Archived from the original (PDF) on 2014-05-14. Retrieved 15 May 2014.
  13. ^ "Hague member countries" (PDF) (in Spanish). cancilleria. Retrieved 15 May 2014.
  14. ^ "Ley 1193 de 2008" (PDF) (in Spanish). Ministerio de Salud, Republica de Colombia. Archived from the original (PDF) on 2010-12-21. Retrieved 15 May 2014.
  15. ^ "Decreto 4192 de 2010" (PDF) (in Spanish). Presidencia, Republica de Colombia. Archived from the original (PDF) on 2014-05-14. Retrieved 15 May 2014.
  16. ^ "Resolución 5549 de 2010" (PDF) (in Spanish). Presidencia, Republica de Colombia. Archived from the original (PDF) on 2013-01-20. Retrieved 15 May 2014.