The cell capsule is a very large structure of some prokaryotic cells, such as bacterial cells. It is a polysaccharide layer that lies outside the cell envelope of bacteria, and is thus deemed part of the outer envelope of a bacterial cell. It is a well-organized layer, not easily washed off, and it can be the cause of various diseases.
The capsule—which can be found in both Gram-negative bacteria and Gram-positive bacteria—should not be confused with the second lipid membrane (or bacterial outer membrane), which contains lipopolysaccharides and lipoproteins and is found only in Gram-negative bacteria. When the amorphous viscid secretion (that makes up the capsule) diffuses into the surrounding medium and remains as a loose undemarcated secretion, it is known as slime layer.
It usually consists of polysaccharides, but can be composed of other materials (e.g., polypeptide(D-glutamic acid) in B. anthracis), also peptidoglycan and muramic acid found in E.coli bacterial capsule. Because most capsules are so tightly packed, they are difficult to stain using standard stains because most stains cannot adhere to the capsule. For examination under the microscope, the bacteria and their background are stained darker than the capsule, which doesn't stain. When viewed, bacterial cells as well as the surface they are on, are stained dark, while the capsule remains pale or colorless and appears as a ring, or halo, around the cell.
The capsule is considered a virulence factor because it enhances the ability of bacteria to cause disease (e.g. prevents phagocytosis). The capsule can protect cells from engulfment by eukaryotic cells, such as macrophages. A capsule-specific antibody may be required for phagocytosis to occur. Capsules also contain water which protects the bacteria against desiccation. They also exclude bacterial viruses and most hydrophobic toxic materials such as detergents. Immunity to one capsule type does not result in immunity to the other types. Capsules also help cells adhere to surfaces.
The capsule is found most commonly among Gram-negative bacteria:
- Escherichia coli (in some strains)
- Neisseria meningitidis
- Klebsiella pneumoniae
- Haemophilus influenzae
- Pseudomonas aeruginosa
- Salmonella
However, some Gram-positive bacteria may also have a capsule:
- Bacillus megaterium for example, synthesizes a capsule composed of polypeptide and polysaccharides.
- Streptococcus pyogenes synthesizes a hyaluronic acid capsule.
- Streptococcus pneumoniae has at least 91 different capsular serotypes. These serotypes are the basis for the pneumococcal vaccines.
- Streptococcus agalactiae produces a polysaccharide capsule of nine antigenic types that all contain sialic acid (Ia, Ib, II, III, IV, V, VI, VII, VIII).
- Staphylococcus epidermidis
A common mnemonic used to remember some encapsulated pathogens is:
"Even Some Super Killers Have Pretty Nice Big Capsules"
Escherichia coli, Streptococcus pneumoniae, Salmonella, Klebsiella pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, Neisseria meningitidis, Bacteroides fragilis, and the yeast Cryptococcus neoformans.
Demonstration of capsuleEdit
- India ink staining: the capsule appears as a clear halo around the bacterium as the ink can't penetrate the capsule.:87
- Maneval's capsule stain: the capsule appears as a clear halo between the pink-stained bacterium and the bluish-grey stained background. The background stain is the acidic stain Congo red (which changes color to bluish-grey due to the pH), and the pink stain is acid fuschin.
- Serological methods: Capsular material is antigenic and can be demonstrated by mixing it with a specific anticapsular serum. When examined under the microscope, the capsule appears 'swollen' due to an increase in its refractivity. This phenomenon is the basis of Quellung reaction.
Use in vaccinationEdit
Vaccination using capsular material is effective against some organisms (e.g., H. influenzae type b, S. pneumoniae, and N. meningitidis). However, polysaccharides are not highly antigenic, especially in children, so many capsular vaccines contain polysaccharides conjugated with protein carriers, such as the tetanus toxoid or diphtheria toxoid. This stimulates a much more robust immune response.
- "bacterial capsule" at Dorland's Medical Dictionary
- Encyclopædia Britannica [Capsules and Slime Layers].
- Daffé, M.; Etienne, G. (1999). "The capsule of Mycobacterium tuberculosis and its implications for pathogenicity". Tubercle and Lung Disease. 79 (3): 153–69. doi:10.1054/tuld.1998.0200. PMID 10656114.
- "Meningococcal meningitis". Textbookofbacteriology.net. Archived from the original on 2014-02-09. Retrieved 2014-01-22.
- Yoshida, K; Matsumoto, T; Tateda, K; Uchida, K; Tsujimoto, S; Yamaguchi, K (November 2000). "Role of bacterial capsule in local and systemic inflammatory responses of mice during pulmonary infection with Klebsiella pneumoniae". Journal of Medical Microbiology. 49 (11): 1003–10. doi:10.1099/0022-1317-49-11-1003. PMID 11073154.
- Schouls, Leo; Van Der Heide, Han; Witteveen, Sandra; Zomer, Bert; Van Der Ende, Arie; Burger, Marina; Schot, Corrie (2008). "Two variants among Haemophilus influenzae serotype b strains with distinct bcs4, hcsA and hcsB genes display differences in expression of the polysaccharide capsule". BMC Microbiology. 8 (1): 35. doi:10.1186/1471-2180-8-35. PMC . PMID 18298818.
- Deretic, V; Dikshit, R; Konyecsni, WM; Chakrabarty, AM; Misra, TK (1989). "The algR gene, which regulates mucoidy in Pseudomonas aeruginosa, belongs to a class of environmentally responsive genes". Journal of Bacteriology. 171 (3): 1278–1283. PMC . PMID 2493441.
- Hyams, Catherine; Camberlein, Emilie; Cohen, Jonathan M.; Bax, Katie; Brown, Jeremy S. (2010-02-01). "The Streptococcus pneumoniae Capsule Inhibits Complement Activity and Neutrophil Phagocytosis by Multiple Mechanisms". Infection and Immunity. 78 (2): 704–715. doi:10.1128/IAI.00881-09. ISSN 0019-9567. PMC . PMID 19948837.
- Gates, Marcellene A.; Thorkildson, Peter; Kozel, Thomas R. (April 1390). "Molecular architecture of the Cryptococcus neoformans capsule". Molecular Microbiology. 52 (1): 13–24. doi:10.1111/j.1365-2958.2003.03957.x. PMID 15049807.
- Prokaryotes. Newnes. April 11, 1996. ISBN 978-0-08-098473-5.
- Goldblatt, D (2000-01-01). "Conjugate vaccines". Clinical and Experimental Immunology. 119 (1): 1–3. doi:10.1046/j.1365-2249.2000.01109.x. ISSN 0009-9104. PMC . PMID 10671089.