The Anchitheriinae are an extinct subfamily of the Perissodactyla family Equidae, the same family which includes modern horses, zebras and donkeys. This subfamily is more primitive than the living members of the family. The group first appeared with Mesohippus in North America during the middle Eocene and thrived until the late Miocene. The subfamily continued in Eurasia with the genus Sinohippus until the early Pliocene, when it finally became extinct. This subfamily were considered browsers with their more ancestral low-crowned molars, in contrast with the modern, specialized grazer species today.

Temporal range: 42.0–3.6 Ma
Middle Eocene to early Pliocene
Scientific classification e
Kingdom: Animalia
Phylum: Chordata
Class: Mammalia
Order: Perissodactyla
Family: Equidae
Subfamily: Anchitheriinae
Leidy, 1869