5'-3' exoribonuclease 2

5'-3' Exoribonuclease 2 (XRN2) also known as Dhm1-like protein is an exoribonuclease enzyme that in humans is encoded by the XRN2 gene.

XRN2
Identifiers
Aliases	XRN2, entrez:22803, 5'-3' exoribonuclease 2
External IDs	OMIM: 608851 MGI: 894687 HomoloGene: 6927 GeneCards: XRN2
Gene location (Human) Chr. Chromosome 20 (human) Band 20p11.22 Start 21,303,331 bp End 21,389,825 bp
Gene location (Mouse) Chr. Chromosome 2 (mouse) Band 2|2 G2 Start 146,854,916 bp End 146,919,920 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in monocyte ganglionic eminence islet of Langerhans smooth muscle tissue Achilles tendon appendix gallbladder stromal cell of endometrium left adrenal gland right adrenal gland Top expressed in yolk sac ganglionic eminence maxillary prominence abdominal wall spermatid seminal vesicula somite neural tube calvaria medial ganglionic eminence More reference expression data BioGPS n/a
Gene ontology Molecular function DNA binding 5'-3' exoribonuclease activity 5'-3' exonuclease activity metal ion binding protein binding nucleic acid binding nuclease activity transcription termination site sequence-specific DNA binding exonuclease activity hydrolase activity 3'-5'-exoribonuclease activity RNA binding Cellular component membrane aggresome nucleolus nucleus nucleoplasm Biological process RNA processing regulation of transcription, DNA-templated DNA-templated transcription, termination mRNA processing RNA catabolic process transcription, DNA-templated DNA catabolic process, exonucleolytic spermatogenesis RNA metabolic process nucleobase-containing compound metabolic process neuron differentiation retina development in camera-type eye rRNA processing RNA phosphodiester bond hydrolysis, exonucleolytic hippocampus development Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 22803 24128 Ensembl ENSG00000088930 ENSMUSG00000027433 UniProt Q9H0D6 Q9DBR1 RefSeq (mRNA) NM_012255 NM_001317960 NM_011917 NM_001356402 NM_001356403 RefSeq (protein) NP_001304889 NP_036387 NP_036047 NP_001343331 NP_001343332 Location (UCSC) Chr 20: 21.3 – 21.39 Mb Chr 2: 146.85 – 146.92 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

The human gene encoding XRN2 shares similarity with the mouse Dhm1 and the yeast's Dhp1 (Schizosaccharomyces pombe) or RAT1 (Saccharomyces) genes. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking and termination. Complementation studies show that Dhm1 has a similar function in mouse as Dhp1.

Function

Human XRN2 is involved in the torpedo model of transcription termination.

The C. elegans homologue, XRN-2, is involved in the degradation of certain mature miRNAs and their dislodging from miRISC miRNAs.

In yeast, the Rat1 protein has been shown to also be involved in the torpedo transcription termination model. When a polyadenylation site has been detected on the nascent RNA and cleaved by the RNA polymerase II, the Rtt103 factor recruits Rat1 and attaches it to free end. The exonuclease activity of Rat1 degrades the RNA strand and halts transcriptions upon catching up to the polymerase.

See also

• Xrn1

External links

• Human XRN2 genome location and XRN2 gene details page in the UCSC Genome Browser.

Further reading

• Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Deloukas P, Matthews LH, Ashurst J, et al. (2001). "The DNA sequence and comparative analysis of human chromosome 20". Nature. 414 (6866): 865–71. Bibcode:2001Natur.414..865D. doi:10.1038/414865a. PMID 11780052.
• Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
• Lu Y, Liu P, James M, et al. (2010). "Genetic variants cis-regulating Xrn2 expression contribute to the risk of spontaneous lung tumor". Oncogene. 29 (7): 1041–9. doi:10.1038/onc.2009.396. PMC 6019621. PMID 19915612.
• Lejeune F, Li X, Maquat LE (2003). "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities". Mol. Cell. 12 (3): 675–87. doi:10.1016/S1097-2765(03)00349-6. PMID 14527413.
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Yang XC, Sullivan KD, Marzluff WF, Dominski Z (2009). "Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing". Mol. Cell. Biol. 29 (1): 31–42. doi:10.1128/MCB.00776-08. PMC 2612496. PMID 18955505.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Scherl A, Couté Y, Déon C, et al. (2002). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
• Kaneko S, Rozenblatt-Rosen O, Meyerson M, Manley JL (2007). "The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination". Genes Dev. 21 (14): 1779–89. doi:10.1101/gad.1565207. PMC 1920172. PMID 17639083.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gromak N, West S, Proudfoot NJ (2006). "Pause sites promote transcriptional termination of mammalian RNA polymerase II". Mol. Cell. Biol. 26 (10): 3986–96. doi:10.1128/MCB.26.10.3986-3996.2006. PMC 1488997. PMID 16648491.
• Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Li J, Zheng H, Ji C, et al. (2005). "A novel splice variant of human XRN2 gene is mainly expressed in blood leukocyte". DNA Seq. 16 (2): 143–6. doi:10.1080/10425170500066771. PMID 16147866. S2CID 35865401.
• Mammalian Gene Collection Program Team, Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proceedings of the National Academy of Sciences. 99 (26): 16899–16903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.

External links

• XRN2+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)