Times from 27 October 2019 up to 29 March 2020 (second legs of round of 16 and first leg of quarter-finals) are CET (UTC+1), all other times are CEST (UTC+2), as listed by UEFA (local times, if different, are in parentheses).
The knockout phase involves 32 teams: 22 teams which received a bye, and the ten teams which advanced from the qualifying round (ten group winners).
Below are the 32 teams which participate in the knockout phase (with their 2019 UEFA women's club coefficients, which take into account their performance in European competitions from 2014–15 to 2018–19 plus 33% of their association coefficient from the same time span).
Each tie in the knockout phase, apart from the final, is played over two legs, with each team playing one leg at home. The team that scores more goals on aggregate over the two legs advance to the next round. If the aggregate score is level, the away goals rule is applied, i.e. the team that scores more goals away from home over the two legs advances. If away goals are also equal, then extra time is played. The away goals rule is again applied after extra time, i.e. if there are goals scored during extra time and the aggregate score is still level, the visiting team advances by virtue of more away goals scored. If no goals are scored during extra time, the tie is decided by penalty shoot-out. In the final, which is played as a single match, if the score is level at the end of normal time, extra time is played, followed by penalty shoot-out if the score remains tied.
The mechanism of the draws for each round is as follows:
In the draw for the round of 32, the sixteen teams with the highest UEFA women's club coefficients are seeded (with the title holders being the automatic top seed), and the other sixteen teams are unseeded. The seeded teams are drawn against the unseeded teams, with the seeded teams hosting the second leg. Teams from the same association or the same qualifying round group cannot be drawn against each other.
In the draw for the round of 16, the eight teams with the highest UEFA women's club coefficients are seeded (with the title holders being the automatic top seed should they qualify), and the other eight teams are unseeded. The seeded teams are drawn against the unseeded teams, with the order of legs decided by draw. Teams from the same association cannot be drawn against each other.
In the draws for the quarter-finals and semi-finals, there are no seedings, and teams from the same association can be drawn against each other. As the draws for the quarter-finals and semi-finals are held together before the quarter-finals are played, the identity of the teams in the semi-finals are not known at the time of the draw. A draw is also held to determine the "home" team for the final (for administrative purposes as it is played at a neutral venue).
The final will be played on 24 May 2020 at the Viola Park in Vienna. The "home" team for the final (for administrative purposes) will be determined by an additional draw held after the quarter-final and semi-final draws.