The 2016 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the twelfth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Guayaquil, Ecuador, between October 31 and November 6, 2016.
| 2016 Challenger Ciudad de Guayaquil | |
|---|---|
| Date | 31 October – 6 November |
| Edition | 12th |
| Surface | Clay |
| Location | Guayaquil, Ecuador |
| Champions | |
| Singles | |
 Nicolás Kicker | |
| Doubles | |
 Ariel Behar /  Fabiano de Paula | |
Singles main-draw entrants edit
Seeds edit
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 POR
|
Gastão Elias | 57 | 1 |
| ARG
|
Facundo Bagnis | 67 | 2 |
| ARG
|
|||
| ARG
|
Carlos Berlocq | 89 | 4 |
 DOM
|
Víctor Estrella Burgos | 94 | 5 |
 COL
|
Santiago Giraldo | 100 | 6 |
| BRA
|
Rogério Dutra Silva | 105 | 7 |
| BRA
|
João Souza | 125 | 8 |
 USA
|
Ernesto Escobedo | 126 | 9 |
- 1 Rankings are as of October 24, 2016.
Other entrants edit
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Daniel Elahi Galán
Bastián Malla
Andrés Molteni
João Pedro Sorgi
The following player entered as a lucky loser:
Champions edit
Singles edit
- Nicolás Kicker def.
Arthur De Greef 6–3, 6–2
Doubles edit
Ariel Behar / Fabiano de Paula def. 
Marcelo Arévalo / 
Sergio Galdós 6–2, 6–4
