First-seeded and reigning champion Pauline Betz defeated second-seeded Margaret Osborne 6–3, 8–6 in the final to win the women's singles tennis title at the 1944 U.S. National Championships. The tournament was played on outdoor grass courts and held from August 30 through September 4, 1944 at the West Side Tennis Club in Forest Hills, Queens, New York.
|1944 U.S. National Championships|
|Final score||6–3, 8–6|
The draw consisted of 32 players of which eight were seeded.
The eight seeded U.S. players are listed below. Pauline Betz is the champion; others show in brackets the round in which they were eliminated.