First-seeded and reigning champion Pauline Betz defeated second-seeded Louise Brough 6–3, 5–7, 6–3 in the final to win the women's singles tennis title at the 1943 U.S. National Championships. The tournament was played on outdoor grass courts and held from September 1 through September 4, 1943 at the West Side Tennis Club in Forest Hills, Queens, New York.
|1943 U.S. National Championships|
|Final score||6–3, 5–7, 6–3|
The draw consisted of 32 players of which eight were seeded.
|5||Sarah Palfrey Cooke||1||3|
- Collins, Bud (2010). The Bud Collins History of Tennis (2nd ed.). New York City: New Chapter Press. p. 470. ISBN 978-0942257700.
- "Lawn Tennis". The West Australian. Vol. 59, no. 17, 834. Western Australia. 6 September 1943. p. 5 – via National Library of Australia.
- "Rankings for National Tennis Championships". The Argus. No. 30, 270. Melbourne. 2 September 1943. p. 9 – via National Library of Australia.