Homeobox protein TGIF1

Homeobox protein TGIF1 is a protein that, in humans, is encoded by the TGIF1 gene. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.

TGIF1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2LK2
Identifiers
Aliases	TGIF1, HPE4, TGIF, TGFB induced factor homeobox 1
External IDs	OMIM: 602630 MGI: 1194497 HomoloGene: 7574 GeneCards: TGIF1
Gene location (Human) Chr. Chromosome 18 (human) Band 18p11.31 Start 3,411,608 bp End 3,459,978 bp
Gene location (Mouse) Chr. Chromosome 17 (mouse) Band 17|17 E1.3 Start 71,151,200 bp End 71,160,541 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in stromal cell of endometrium gallbladder corpus epididymis rectum gastric mucosa right uterine tube left uterine tube skin of abdomen caput epididymis minor salivary glands Top expressed in saccule ectoderm otic placode lip primitive streak corneal stroma spermatocyte cumulus cell abdominal wall maxillary prominence More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein binding DNA-binding transcription factor activity RNA polymerase II cis-regulatory region sequence-specific DNA binding DNA-binding transcription repressor activity, RNA polymerase II-specific transcription corepressor activity DNA-binding transcription factor activity, RNA polymerase II-specific co-SMAD binding Cellular component nucleus nucleoplasm Biological process regulation of transcription, DNA-templated negative regulation of gene expression multicellular organism development cellular response to growth factor stimulus negative regulation of transcription by RNA polymerase II transcription, DNA-templated Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7050 21815 Ensembl ENSG00000177426 ENSMUSG00000047407 UniProt Q15583 P70284 RefSeq (mRNA) NM_001278682 NM_001278684 NM_001278686 NM_003244 NM_170695 NM_173207 NM_173208 NM_173209 NM_173210 NM_173211 NM_174886 NM_001374396 NM_001374397 NM_001164074 NM_001164075 NM_001164076 NM_001164077 NM_009372 RefSeq (protein) NP_001265611 NP_001265613 NP_001265615 NP_003235 NP_733796 NP_775299 NP_775300 NP_775301 NP_775302 NP_775303 NP_777480 NP_001361325 NP_001361326 NP_001157546 NP_001157547 NP_001157548 NP_001157549 NP_033398 Location (UCSC) Chr 18: 3.41 – 3.46 Mb Chr 17: 71.15 – 71.16 Mb PubMed search
Wikidata
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Function

The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult.

Clinical significance

Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. It has also been associated with risk of otitis media (inflammation of the middle ear)

Interactions

Homeobox protein TGIF1 has been shown to interact with:

• C-jun,
• CTBP1,
• HDAC1, and
• Mothers against decapentaplegic homolog 2.

Further reading

• El-Jaick KB, Powers SE, Bartholin L, Myers KR, Hahn J, Orioli IM, Ouspenskaia M, Lacbawan F, Roessler E, Wotton D, Muenke M (2007). "Functional analysis of mutations in TGIF associated with holoprosencephaly". Mol. Genet. Metab. 90 (1): 97–111. doi:10.1016/j.ymgme.2006.07.011. PMC 1820763. PMID 16962354.
• Münke M, Page DC, Brown LG, Armson BA, Zackai EH, Mennuti MT, Emanuel BS (1989). "Molecular detection of a Yp/18 translocation in a 45,X holoprosencephalic male". Hum. Genet. 80 (3): 219–23. doi:10.1007/BF01790089. PMID 3192211. S2CID 15442879.
• Overhauser J, Mitchell HF, Zackai EH, Tick DB, Rojas K, Muenke M (1995). "Physical mapping of the holoprosencephaly critical region in 18p11.3". Am. J. Hum. Genet. 57 (5): 1080–5. PMC 1801375. PMID 7485158.
• Wotton D, Lo RS, Lee S, Massagué J (1999). "A Smad transcriptional corepressor". Cell. 97 (1): 29–39. doi:10.1016/S0092-8674(00)80712-6. PMID 10199400. S2CID 6907878.
• Yang Y, Hwang CK, D'Souza UM, Lee SH, Junn E, Mouradian MM (2000). "Three-amino acid extension loop homeodomain proteins Meis2 and TGIF differentially regulate transcription". J. Biol. Chem. 275 (27): 20734–41. doi:10.1074/jbc.M908382199. PMID 10764806.
• Melhuish TA, Wotton D (2001). "The interaction of the carboxyl terminus-binding protein with the Smad corepressor TGIF is disrupted by a holoprosencephaly mutation in TGIF". J. Biol. Chem. 275 (50): 39762–6. doi:10.1074/jbc.C000416200. PMID 10995736.
• Lo RS, Wotton D, Massagué J (2001). "Epidermal growth factor signaling via Ras controls the Smad transcriptional co-repressor TGIF". EMBO J. 20 (1–2): 128–36. doi:10.1093/emboj/20.1.128. PMC 140192. PMID 11226163.
• Pessah M, Prunier C, Marais J, Ferrand N, Mazars A, Lallemand F, Gauthier JM, Atfi A (2001). "c-Jun interacts with the corepressor TG-interacting factor (TGIF) to suppress Smad2 transcriptional activity". Proc. Natl. Acad. Sci. U.S.A. 98 (11): 6198–203. Bibcode:2001PNAS...98.6198P. doi:10.1073/pnas.101579798. PMC 33445. PMID 11371641.
• Yu X, Li P, Roeder RG, Wang Z (2001). "Inhibition of androgen receptor-mediated transcription by amino-terminal enhancer of split". Mol. Cell. Biol. 21 (14): 4614–25. doi:10.1128/MCB.21.14.4614-4625.2001. PMC 87125. PMID 11416139.
• Wotton D, Knoepfler PS, Laherty CD, Eisenman RN, Massagué J (2001). "The Smad transcriptional corepressor TGIF recruits mSin3". Cell Growth Differ. 12 (9): 457–63. PMID 11571228.
• Sharma M, Sun Z (2002). "5'TG3' interacting factor interacts with Sin3A and represses AR-mediated transcription". Mol. Endocrinol. 15 (11): 1918–28. doi:10.1210/mend.15.11.0732. PMID 11682623.
• Dintilhac A, Bernués J (2002). "HMGB1 interacts with many apparently unrelated proteins by recognizing short amino acid sequences". J. Biol. Chem. 277 (9): 7021–8. doi:10.1074/jbc.M108417200. hdl:10261/112516. PMID 11748221.
• Chen CP, Chern SR, Du SH, Wang W (2002). "Molecular diagnosis of a novel heterozygous 268C-->T (R90C) mutation in TGIF gene in a fetus with holoprosencephaly and premaxillary agenesis". Prenat. Diagn. 22 (1): 5–7. doi:10.1002/pd.202. PMID 11810641. S2CID 8297098.
• Aguilella C, Dubourg C, Attia-Sobol J, Vigneron J, Blayau M, Pasquier L, Lazaro L, Odent S, David V (2003). "Molecular screening of the TGIF gene in holoprosencephaly: identification of two novel mutations". Hum. Genet. 112 (2): 131–4. doi:10.1007/s00439-002-0862-8. PMID 12522553. S2CID 2238304.
• Chen F, Ogawa K, Nagarajan RP, Zhang M, Kuang C, Chen Y (2003). "Regulation of TG-interacting factor by transforming growth factor-beta". Biochem. J. 371 (Pt 2): 257–63. doi:10.1042/BJ20030095. PMC 1223316. PMID 12593671.
• Lam DS, Lee WS, Leung YF, Tam PO, Fan DS, Fan BJ, Pang CP (2003). "TGFbeta-induced factor: a candidate gene for high myopia". Invest. Ophthalmol. Vis. Sci. 44 (3): 1012–5. doi:10.1167/iovs.02-0058. PMID 12601022.
• Dubourg C, Lazaro L, Pasquier L, Bendavid C, Blayau M, Le Duff F, Durou MR, Odent S, David V (2004). "Molecular screening of SHH, ZIC2, SIX3, and TGIF genes in patients with features of holoprosencephaly spectrum: Mutation review and genotype-phenotype correlations". Hum. Mutat. 24 (1): 43–51. doi:10.1002/humu.20056. PMID 15221788. S2CID 34076824.