Shine-Dalgarno sequence
The Shine-Dalgarno sequence (or Shine-Dalgarno box), proposed by Australian scientists John Shine (b.1946) and Lynn Dalgarno (b.1935),[1] is a ribosomal binding site in the mRNA, generally located 8 bases upstream of the start codon AUG. The Shine-Dalgarno sequence exists both in bacteria and archaea, being also present in some chloroplastic and mitochondial transcripts. The six-base consensus sequence is AGGAGG; in E. coli, for example, the sequence is AGGAGGU. This sequence helps recruit the ribosome to the mRNA to initiate protein synthesis by aligning it with the start codon. The complementary sequence (UCCUCC), is called the anti-Shine-Dalgarno sequence and is located at the 3' end of the 16S rRNA in the ribosome. The eukaryotic equivalent of the Shine-Dalgarno sequence is called the Kozak sequence.
Mutations in the Shine-Dalgarno sequence can reduce or increase[2]translation. This change is due to a reduced or increased mRNA-ribosome pairing efficiency, as evidenced by the fact that complementary mutations in the anti-Shine-Dalgarno sequence can restore translation.
The Shine-Dalgarno sequence GAGG dominates in bacteriophage T4 early genes, whereas the sequence GGAG is a target for the T4 endonuclease RegB that initiates the early mRNA degradation.[3]
When the Shine-Dalgarno sequence and anti-Shine-Dalgarno sequence pair, the translation initiation factors IF2-GTP, IF1, IF3, as well as the initiator tRNA fMet-tRNA(fmet) are recruited to the ribosome.
The ribosomal S1 protein in Gram-negative bacteria
In Gram-negative bacteria, the presence of a Shine-Dalgarno sequence is not obligatory for the ribosome to locate the initiator codon. Numerous prokaryotic mRNAs don't possess Shine-Dalgarno sequences at all: ribosomal protein S1, which binds to AU-rich sequences found in many prokaryotic mRNAs 15-30 nucleotides upstream of start-codon, can instigate translation initiation in the case of these mRNAs.
SD-Sequences in Chloroplasts
Although plastids are prokaryotic descendants and still have their prokaryotic translational machinery, SD-like sequences are not required at least in green alga Chlamydomonas reinhardtii chloroplasts according to a study.[4]
See also
- Kozak consensus sequence, the sequence that targets the ribosome to the initiation codon in eukaryotes.
- Prokaryotic translation
References
- ^ Shine J, Dalgarno L (1975). "Determinant of cistron specificity in bacterial ribosomes". Nature 254 (5495): 34–8. doi:10.1038/254034a0. PMID 803646.
- ^ Johnson G (1991). "Interference with phage lambda development by the small subunit of the phage 21 terminase, gp1". Journal of Bacteriology 173 (9): 2733–2738. PMID 1826903.
- ^ Malys N (2012). "Shine-Dalgarno sequence of bacteriophage T4: GAGG prevails in early genes". Molecular Biology Reports 39 (1): 33–9. doi:10.1007/s11033-011-0707-4. PMID 21533668.
- ^ Fargo DC, Zhang M, Gillham NW, Boynton JE. (1998). "Shine-Dalgarno-like sequences are not required for translation of chloroplast mRNAs in Chlamydomonas reinhardtii chloroplasts or in Escherichia coli". Mol Gen Genet 257 (3): 271–82. PMID 9520261.
Further reading
- Voet D and Voet J (2004). Biochemistry (3rd ed.). John Wiley and Sons Inc. pp. 1321–1322 and 1342–1343.
- Malys N, McCarthy JEG (2011). "Translation initiation: variations in the mechanism can be anticipated". Cellular and Molecular Life Sciences 68 (6): 991–1003. doi:10.1007/s00018-010-0588-z. PMID 21076851. Unknown parameter
|unused_data=ignored (help) - Mustafa Cicek, Ozal Mutlu, Aysegul Erdemir, Ebru Ozkan, Yunus Saricay, Dilek Turgut-Balik (2013), "Single Mutation in Shine-Dalgarno-Like Sequence Present in the Amino Terminal of Lactate Dehydrogenase of Plasmodium Effects the Production of an Eukaryotic Protein Expressed in a Prokaryotic System". Molecular Biotechnology 54(2): 602-608. http://link.springer.com/article/10.1007/s12033-012-9602-z
External links
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