|Pou domain - N-terminal to homeobox domain|
The acronym POU is derived from the names of three transcription factors:
There is a surprisingly high degree of amino acid sequence conservation (37%-42%) of POU homeodomains to the transcriptional regulator comS from the gram positive prokaryote Bacillus subtilis. Interestingly, akin to the way that POU homeodomain regulators lead to tissue differentiation in metazoans, this transcription factor is critical for differentiation of a subpopulation of B. subtilis into a state of genetic competence.
POU proteins are eukaryotic transcription factors containing a bipartite DNA binding domain referred to as the POU domain. The acronym POU (pronounced 'pow') is derived from the names of three transcription factors, the pituitary-specific Pit-1, the octamer-binding proteins Oct-1 and Oct-2, and the neural Unc-86 from Caenorhabditis elegans. POU domain genes have been described in organisms as divergent as Caenorhabditis elegans, Drosophila, Xenopus, zebrafish and human but have not been yet identified in plants and fungi. The various members of the POU family have a wide variety of functions, all of which are related to the function of the neuroendocrine system and the development of an organism. Some other genes are also regulated, including those for immunoglobulin light and heavy chains (Oct-2), and trophic hormone genes, such as those for prolactin and growth hormone (Pit-1).
The POU domain is a bipartite domain composed of two subunits separated by a non-conserved region of 15-55 aa. The N-terminal subunit is known as the POU-specific (POUs) domain (IPR000327), while the C-terminal subunit is a homeobox domain (IPR007103). 3D structures of complexes including both POU subdomains bound to DNA are available. Both subdomains contain the structural motif 'helix-turn-helix', which directly associates with the two components of bipartite DNA binding sites, and both are required for high affinity sequence-specific DNA-binding. The domain may also be involved in protein-protein interactions. The subdomains are connected by a flexible linker. In proteins a POU-specific domain is always accompanied by a homeodomain. Despite of the lack of sequence homology, 3D structure of POUs is similar to 3D structure of bacteriophage lambda repressor and other members of HTH_3 family.
Human genes encoding proteins containing the POU domain include:
- Phillips K, Luisi B (2000). "The virtuoso of versatility: POU proteins that flex to fit". J. Mol. Biol. 302 (5): 1023–39. doi:10.1006/jmbi.2000.4107. PMID 11183772.
- D'Souza C, Nakano MM, Zuber P (September 1994). "Identification of comS, a gene of the srfA operon that regulates the establishment of genetic competence in Bacillus subtilis". Proc. Natl. Acad. Sci. U.S.A. 91 (20): 9397–401. doi:10.1073/pnas.91.20.9397. PMC 44819. PMID 7937777.
- Assa-Munt N, Mortishire-Smith RJ, Aurora R, Herr W, Wright PE (1993). "The solution structure of the Oct-1 POU-specific domain reveals a striking similarity to the bacteriophage lambda repressor DNA-binding domain". Cell 73 (1): 193–205. doi:10.1016/0092-8674(93)90171-L. PMID 8462099.
- Rosenfeld MG, Andersen B (2001). "POU domain factors in the neuroendocrine system: lessons from developmental biology provide insights into human disease". Endocr. Rev. 22 (1): 2–35. doi:10.1210/er.22.1.2. PMID 11159814.
- Petryniak B, Thompson CB, Staudt LM, Postema CE, McCormack WT (1990). "Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution". Proc. Natl. Acad. Sci. U.S.A. 87 (3): 1099–1103. doi:10.1073/pnas.87.3.1099. PMC 53418. PMID 1967834.
- Hirsh J, Johnson WA (1990). "Binding of a Drosophila POU-domain protein to a sequence element regulating gene expression in specific dopaminergic neurons". Nature 343 (6257): 467–470. doi:10.1038/343467a0. PMID 1967821.
- Mathis JM, Simmons DM, He X, Swanson LW, Rosenfeld MG (1992). "Brain 4: a novel mammalian POU domain transcription factor exhibiting restricted brain-specific expression". EMBO J. 11 (7): 2551–2561. PMC 556730. PMID 1628619.
- Phillips K, Luisi B (2000). "The virtuoso of versatility: POU proteins that flex to fit". J. Mol. Biol. 302 (5): 1023–1039. doi:10.1006/jmbi.2000.4107. PMID 11183772.
- Pabo CO, Aurora R, Herr W, Klemm JD, Rould MA (1994). "Crystal structure of the Oct-1 POU domain bound to an octamer site: DNA recognition with tethered DNA-binding modules". Cell 77 (1): 21–32. doi:10.1016/0092-8674(94)90231-3. PMID 8156594.
- Rosenfeld MG, Aggarwal AK, Li P, Jacobson EM, Leon-del-Rio A (1997). "Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility". Genes Dev. 11 (2): 198–212. doi:10.1101/gad.11.2.198. PMID 9009203.
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