Macarena may refer to:
- "La Macarena", the title given to "Nuestra Señora de la Esperanza" (Our Lady of Hope) (or Mary, the mother of Jesus), in Seville, Spain
- "Macarena", a common first name in Spain (especially in Sevilla) and some Latin American countries
- Nuestra Señora de la Esperanza de la Macarena ("Our Lady of Hope"), a church in Macarena, Seville
- Macarena, Seville, a neighborhood in Seville, Spain, where "La Macarena" is located
- Serranía de la Macarena, a range of mountains in Colombia
- Parque Nacional de La Macarena, a National Park in Colombia in the Serranía de la Macarena
- La Macarena, Colombia, a village in Meta Department, Colombia
- "Macarena" (song), a song by Sevilla natives Los del Río and the dance devised for it
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